Determination of imazalil and thiabendazole by UPLC- Q-TOF-MS – an analysis of a grapefruit extract-containing dietary supplement
DOI:
https://doi.org/10.12923/j.2084-980X/25.4/a.13Keywords:
fungicides, imazalil, thiabendazole, Q-TOF, dietary supplementAbstract
The report presents a method for determining imazalil and thiabendazole in a liquid grapefruit extract-containing dietary supplement using the ultra performance liquid chromatography coupled to quadrupole-time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) system. The employment of hybrid quadrupole coupled with time-of-flight analyzer characterized by a very high resolution allows for identifying the investigated compounds and for employing the developed technique in routine quality control of natural extracts-containing foodstuffs. The limit of detection for imazalil was 0.25 ng/ml, while for thiabendazole its value was 2 ng/ml. The recovery of the investigated fungicides was within the range of 84-109 %. In the studied dietary supplement, the investigators demonstrated trace amounts of imazalil and thiabendazole, what may be explained by biocide diffusion into the flesh of fruits employed in manufacturing products of this type.
References
1. Ibáñez M. et al.: Rapid non-target screening of organic pollutants in water by ultraperformance liquid chromatography coupled to time-of-light mass spectrometry. TrAC, Trends Anal. Chem., 27, 481, 2008.
2. Kruve A. et al.: Pesticide residues in commercially available oranges and evaluation of potential washing methods. Proc. Estonian Acad. Sci. Chem., 56, 134, 2007.
3. Kuster M., de Alda M.L., Barceló D.: Liquid chromatography–tandem mass spectrometric analysis and regulatory issues of polar pesticides in natural and treated waters. J Chromatogr A, 1216, 520, 2009.
4. Núñez O., Moyano E., Galceran M.T.: LC–MS/MS analysis of organic toxics in food. TrAC, Trends Anal. Chem., 24, 683, 2005.
5. Ortelli D., Edder P., Corvi C.: Multiresidue analysis of 74 pesticides in fruits and vegetables by liquid chromatography–electrospray–tandem mass spectrometry. Anal. Chim. Acta, 520, 33, 2004.
6. Picó Y. et al.: Identification of unknown pesticides in fruits using ultra-performance liquid chromatography–quadrupole time-of-flight mass spectrometry: Imazalil as a case study of quantification. J Chromatogr A, 1176, 123, 2007.
7. Picó Y. et al.: Profiling of compounds and degradation products from the postharvest treatment of pears and apples by ultra-high pressure liquid chromatography quadrupole-time-of-flight mass spectrometry. Talanta, 81, 281, 2010.
8. Soler C., Mañes J., Picó Y.: Determination of carbosulfan and its metabolites in oranges by liquid chromatography ion-trap triple-stage mass spectrometry. J Chromatogr A, 1109, 228, 2006.
9. Sullivan D., Crowley R.: Development and validation of analytical methods for dietary supplements. Toxicology, 221, 28, 2006.
10. Takino M., Daishima S., Nakahara T.: Liquid chromatography/mass spectrometric determination of patulin in apple juice using atmospheric pressure photoionization. Rapid Commun. Mass Spectrom., 17, 1965, 2003.
11. Thurman E.M. et al.: Discovering metabolites of post-harvest fungicides in citrus with liquid chromatography/time-of-flight mass spectrometry and ion trap tandem mass spectrometry. J Chromatogr A, 1082, 71, 2005.
12. Yoshioka N. et al.: Rapid determination of five post-harvest fungicides and metabolite in citrus fruits by liquid chromatography/time-of-flight mass spectrometry with atmospheric pressure photoionization. Food Control, 21, 212, 2010.
13. Yoshioka N., Akiyama Y., Teranishi K.: Rapid simultaneous determination of o-phenylphenol, diphenyl, thiabendazole, imazalil and its major metabolite in citrus fruits by liquid chromatography-mass spectrometry using atmospheric pressure photoionization. J Chromatogr A, 1022, 145, 2004.
14. Zamora T. et al.: Determination of tridemorph and other fungicide residues in fruit samples by liquid chromatography–electrospray tandem mass spectrometry. J Chromatogr A, 1045, 137, 2004.
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