Concentrations of free light chains determined by nephelometry and turbidimetry
DOI:
https://doi.org/10.12923/j.2084-980X/25.4/a.20Keywords:
free light chains, nephelometry, turbidimetry, monoclonal gammapathyAbstract
Determination of FLCs in the blood serum has been useful to diagnose patients with monoclonal gammapathies. Besides, the test has been widely used to assess response to treatment and detect progressive conditions. In the course of gammapathy monoclonal light chains constitute homogenic population of chains made of kappa and lambda light immunoglobulins produced by malignant plasmocytes and/or B lymphocytes. Quantitative analysis of FLC by nephelometry and turbidimetry uses specific antibodies which recognize antigenic determiners of light chains. The purpose of our investigation was to compare nephelometric and turbidimetric methods applied in FLC determinations. Comparative determinations were done in the group of 64 patients suspected of monoclonal gammapathy: 30 females (mean age 66yrs) and 34 males (mean age 64yrs). The analysis of results let conclude that nephelometric and turbidimetric techniques applied to determine kappa and lambda free light chains have comparable diagnostic value.
References
1. Abraham R.S., Katzmann J.A., Clark R.J. et.al.: Quantitative analysis of serum free light chains. A new marker for the diagnostic evaluation of primary systemic amyloidosis. Am. J. Clin. Pathol., 119, 274, 2003.
2. Bradwell A.R., Carr-Smith H.D., Mead G.P. et.al.: Highly sensitive, automated immunoassay for immunoglobulin free light chains in serum and urine. Clin. Chem., 47, 673, 2001.
3. Bradwell A.R., Carr-Smith H.D., Mead G.P. et.al.: Serum test for assessment of patients with Bence Jones myeloma. Lancet 361, 489, 2003.
4. Bradwell A.R.: Serum Free Light Chain Analysis. 6th ed. Bradwell 2010.
5. Drayson M., Tang L.X., Drew R. et. al.: Serum free light-chain measurements for identifying and monitoring patients with nonsecretory multiple myeloma. Blood 97, 2900, 2001.
6. Jurczyszyn A., Skotnicki A.B.: Szpiczak mnogi – kompleksowa diagnostyka i terapia, Wgórnicki wydawnictwo medyczne, 2010.
7. Katzmann J.A., Abraham R.S., Dispenzieri A. et.al.: Diagnostic performance of quantitative kappa and lambda free light chain assays in clinical practice. Clin. Chem., 51, 878, 2005.
8. Katzmann J.A., Clark R.J., Abraham R.S. et. al.: Serum reference intervals and diagnostic ranges for free kappa and free lambda immunoglobulin light chains: relative sensitivity for detection of monoclonal light chains. Clin. Chem., 48, 1437, 2002.
9. Kendziorek A., Bobilewicz D.M.: Badania laboratoryjne w różnych stadiach rozwoju gammapatii monoklonalnych. In vitro Explorer 1, 3, 2007.
10. Levinson S.S., Keren D.F.: Free light chains of immunoglobulins: clinical laboratory analysis. Clin. Chem., 40, 1869, 1994.
11. Matters D.J., Solanki M., Jones R. et.al.: Statistical analysis of free light chain results between eight nephelometric and turbidimetric platforms. Hematology meeting reports 2, A5a, 2008.
12. Russo L.M., Bakris G.L., Comper W.D.: Renal handling of albumin: a critical review of basic concepts and perspective. Am. J. Kidney Dis., 39, 899, 2002.
13. Usnarska-Zubkiewicz L., Hołojda J., Kuliczkowski K.: Serum free light chain (sFLC) – diagnostic and prognostic value in plasma cell dyscrasias. Acta Haematol. Pol., 40, 349, 2009.
Downloads
Published
Issue
Section
License
Copyright (c) 2012 Author
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.
