The role of leptin in obesity induced nitrooxidative stress and endothelial dysfunction – nanomedical approach

Authors

  • Mykhaylo Korda I.Ya.Horbachevsky Ternopil State Medical University, Department of Medical Biochemistry Author
  • Svitlana Yavorska I.Ya.Horbachevsky Ternopil State Medical University, Department of Medical Biochemistry Author
  • Tetyana Yaroshenko I.Ya.Horbachevsky Ternopil State Medical University, Department of Medical Biochemistry Author
  • Oksana Ostrivka I.Ya.Horbachevsky Ternopil State Medical University, Department of Medical Biochemistry Author
  • Svitlana Pidruchna I.Ya.Horbachevsky Ternopil State Medical University, Department of Medical Biochemistry Author
  • Nataliya Suslova I.Ya.Horbachevsky Ternopil State Medical University, Department of Medical Biochemistry Author
  • Iryna Kuz’mak I.Ya.Horbachevsky Ternopil State Medical University, Department of Medical Biochemistry Author

Keywords:

obesity, leptin, endothelial dysfunction, nitric oxide, superoxide, peroxynitrite

Abstract

Hyperleptinemia accompanying obesity affects endothelial NO and is a serious factor for vascular disorders. NO, O2-, and ONOO- levels in HUVECs and aortic endothelium of obese mice were recorded using tandem electrochemical nanosensors. 12-h exposure of HUVECs to leptin increased eNOS expression and decreased the [NO]/[ONOO-] ratio. In obese mice, a 2.5-fold increase in leptin concentration coincided with 100% increase in eNOS and about 30% decrease in intracellular L-arginine. This led to eNOS uncoupling, a reduction in bioavailable NO, and an elevated concentration of O2- and ONOO-. L-Arginine supplementation reversed eNOS uncoupling and partially restored [NO]/[ONOO-] balance in obese mice.

References

1. Fenster C.P, Weinsier R.L. et al.: Obesity, aerobic exercise, and vascular disease: the role of oxidant stress. Obes. Res., 10, 964, 2002.

2. Huk I., Nanobashvili J. et al.: L-Arginine treatment alters the kinetics of nitric oxide and superoxide release and reduces ischemia-reperfusion injury in skeletal muscle. Circulation, 96, 667, 1997.

3. Malinski T., Taha Z.: Nitric oxide release from a single cell measured in situ by a porphyrinic based microsensor. Nature, 358, 676, 1992

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Published

2025-04-09

Issue

Vol. 23 No. 2 (2010): Annales UMCS, Pharmacia, Sectio DDD, Volume XXIII

Section

Artykuły

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