Blockade of the endoplasmic reticulum stress sensor inositol requiring enzyme-1 changes the expression of cyclin and growth arrest-specific genes in glioma cells

Authors

  • Dmytro Minchenko Palladin Institute of Biochemistry National Academy of Science of Ukraine, Department of Molecular Biology, Kyiv, Ukraine Author
  • Olena Hubenya Palladin Institute of Biochemistry National Academy of Science of Ukraine, Department of Molecular Biology, Kyiv, Ukraine Author
  • Bohdan Terletsky Palladin Institute of Biochemistry National Academy of Science of Ukraine, Department of Molecular Biology, Kyiv, Ukraine Author
  • Anastasiya Kuznetsova Palladin Institute of Biochemistry National Academy of Science of Ukraine, Department of Molecular Biology, Kyiv, Ukraine Author
  • Michel Moenner INSERM U920 Molecular Mechanisms of Angiogenesis Laboratory, University Bordeaux 1, Talence, France Author
  • Oleksandr Minchenko 1. Palladin Institute of Biochemistry National Academy of Science of Ukraine; 2. Department of Molecular Biology, Kyiv, Ukraine; 3. INSERM U920 Molecular Mechanisms of Angiogenesis Laboratory, University Bordeaux 1, Talence, France Author

DOI:

https://doi.org/10.12923/

Keywords:

inositol requiring enzyme-1, gene expression, cyclin A2, D3, E2 and G2, cyclin-dependent kinase CDK4, growth arrest-specific genes GAS1 and GAS6, glioma cells

Abstract

We studied effect of inositol requiring enzyme-1 (IRE-1) deficiency on the expression of different cyclin and cyclin-dependent kinase (CDK) genes as well as growth arrest-specific (GAS) genes in glioma cells and modified glioma cells without IRE-1 kinase and ribonuclease activities. Cyclin A2, D3, E2 and G2 mRNA expression was significantly increased in IRE-1-deficient glioma cells as compared to control cell line. Blockade of IRE-1 activities significantly induced the expression of growth arrest-specific genes GAS1 and GAS6. Results of these investigations clearly demonstrated that the expression of growth arrest-specific genes and different cyclins in glioma cells significantly depends on IRE-1 kinase and ribonuclease activities.

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Published

2010-09-30

Issue

Vol. 23 No. 3 (2010): Annales UMCS, Pharmacia, Sectio DDD, Volume XXIII

Section

Artykuły

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