Two-component biocomposite: chitosan – hydroxyapatite. Preparation and estimation of properties.
DOI:
https://doi.org/10.12923/Keywords:
hydroxyapatite, chitosan, antibiotic immobilization, implant infection, gentamicinAbstract
Forming a biofilm on orthopedic implant surface is a serious problem nowadays. This research was focused on making new type of biocomposite with antimicrobial properties. Biocomposite was protected from bacterial adhesion (Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25992) and biofilm formation. Biomaterial hybrid showed prolonged antimicrobial activity (25 – 40 days). HAp-chitosan-gentamicin granules were resistant to sterilization process and saved its antimicrobial activity. The possibility of forming different shapes from chitosan-modified HAp granules make this biocomposite promising for future medical applications.
References
1. Dash A.K., Cudworth II G.C.: Therapeutic Applications of Implantable Drug Delivery Systems. J. Pharm. Tox. Meth., 40, 1, 1998
2. Friess W., Schlapp M.: Sterilization of gentamicin containing collagen/PLGA microparticle composites. Eur. J. Pharm. Biopharm., 63, 2, 176, 2006
3. Frutos Cabanillas P., Dièz Peña E., Barrales-Rienda I.M., Frutos G.: Validation and in vitro characterization of antibiotic-loaded bone cement release. Int. J. Pharm., 209, 15, 2000
4. Hench L.L.: Bioceramics. J. Am. Ceram. Soc., 81, 1705, 1998
5. Hu S.G, Jou C.H, Yang M.C.: Protein adsorption, fibroblast activity and antibacterial properties of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) grafted with chitosan and chitooligosaccharide after immobilized with hyaluronic acid. Biomaterials, 24, 2685, 2003
6. Ginalska G., Uryniak A., Łobarzewski J., Osińska M.: Immobilization method of antibiotics contain primary amino groups on solid matrices covered by protein. Polish Patent no P-358934, 2008
7. Ginalska G., Kowalczuk D., Osińska M.: Amikacin-loaded vascular prothesis as an effective drug carrier. Int. J. Pharmaceut., 339, 1-2, 39, 2007
8. Osińska-Jaroszuk M, Ginalska G, Belcarz A, Uryniak A.: Vascular prostheses with covalently bound gentamicin and amikacin reveal superior antibacterial properties than silver-impregnated ones--an in vitro study. Eur. J. Vasc. Endovasc. Surg., 38, 697, 2009
9. Pramanik N., Mishra D., Banerjee I. et al.: Chemical synthesis, characterization and biocompatibility study of hydroxyapatite/chitosan phosphate nanocomposite for bone tissue engineering applications. Int. J. Biomater., 1, 1, 2009
10. Różalska B., Sadowska B., Więckowska M., Rudnicka W.: Wykrywanie biofilmu bakteryjnego na biomateriałach medycznych. Med. Dośw. Mikrobiol., 50, 115, 1998
11. Schmidt G.L., Altman G.T.: Biofilms in orthopaedic surgery. Operative Techniques in Orthopaedics, 12, 4, 242, 2002
12. Shi C., Zhu Y., Ran X. et al.: Therapeutic potential of chitosan and its derivatives in regenerative medicine. J. Surg. Res., 133, 2, 185, 2006
13. Sivakumar M, Rao K.P.: Preparation, characterization, and in vitro release of gentamicin from coralline hydroxyapatite-alginate composite microspheres. J. Biomed. Mater. Res. A., 65, 2, 222, 2003
14. Wang S.: Ordered mesoporous materials for drug delivery. Micro. Meso. Mat., 117, 1, 2009
15. Weetall H.H.: Covalent coupling methods for inorganic support materials. Meth. Enzymol., 44, 134, 1976
16. Wojtasz-Pająk A.: Chitosan dicarboxylic acid salts. In: Progress on chemistry of chitin and its derivatives, 13, 25, 2008
17. Zalewska J., Ginalska G., Belcarz A. et al.: Brzana W., Ślósarczyk A. Optimization of amikacin binding process to modified hydroxyapatite. Engineering of Biomaterials, 10, 69, 96, 2007
18. Zalewska J. Ginalska G.: Amikacin-modified hybrid biomaterial antimicrobial properties. Ann. UMCS, sect. DDD, 22, 2, 5, 39
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