Dietary intake of polyphenolic compoundsfrom major food groups: results of analytical determinations

Authors

DOI:

https://doi.org/10.12923/

Keywords:

food, DPPH, LC-MS, polyphenolic compounds, Folin-Ciocalteu method

Abstract

Polyphenols are widely distributed in plants and plant-based foods. Although they are not considered essential nutrients, they exhibit a broad spectrum of biological properties, particularly antioxidant activity. The aim of this study was to evaluate dietary intake 
of polyphenolic compounds derived from major food groups among young Polish adults. Dietary intake was assessed using a 24-hour dietary recall method supported by dedicated computer software. Liquid chromatography-mass spectrometry (LC-MS) was applied to identify major polyphenolic compounds, while the Folin-Ciocalteu assay and the DPPH radical scavenging test were used to evaluate the antioxidant activity of basic food groups. The primary sources of polyphenolic compounds were fruits, vegetables, and, most notably, beverages – mainly tea – which accounted for over 65% of beverage consumption in both interviewed groups. The predominant phenolic compounds identified were gallic acid, epigallocatechin gallate (EGCG), quercetin, and rutin. Gallic acid was the main polyphenolic component in daily food rations, with beverages constituting its primary dietary source. Only trace amounts of polyphenols were detected in eggs, fish, oils, milk, and meat products. The antioxidant activity of female diets (682.6 mg GAEq) was slightly higher compared with male food rations (680.04 mg GAEq).

Author Biography

  • Wojciech Koch, Department of Food and Nutrition, Medical University of Lublin, Poland

        

References

1. Chen Y, Michalak M, Agellon LB. Importance of nutrients and nutrient metabolism on human health. Yale J Biol Med. 2018;91(2):95-103.

2. Cheynier V. Phenolic compounds: from plants to foods. Phytochem Rev. 2012;11:153-177.

3. Tomás-Barberán FA, Ferreres F, Gil MI. Antioxidant phenolic metabolites from fruit and vegetables and changes during postharvest storage and processing. Stud Nat Prod Chem. 2000;23:739-795.

4. Bendary E, Francis RR, Ali HMG, Sarwat MI, El Hady S. Antioxidant and structure-activity relationships (SARs) of some phenolic and aniline compounds. Ann Agric Sci. 2013;58(2):173-181.

5. Jopkiewicz S. Oxidative stress. Part I. Oxidative stress as a factor in the development of civilization diseases. Med Śr. 2018;21(2):48-52.

6. Madamanchi NR, Vendrov A, Runge MS. Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol. 2005;25(1):29-38.

7. Zajdel A, Wilczok A, Słowiński J, Orchel J, Mazurek U. Aldehydic lipid peroxidation products in human brain astrocytomas. J Neurooncol. 2007;84(2):167-173.

8. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44-84.

9. Adebooye OC, Vijayalakshmi R, Singh V. Peroxidase activity, chlorophylls and antioxidant profile of two leaf vegetables (Solanum nigrum L. and Amaranthus cruentus L.) under six pretreatment methods before cooking. Int J Food Sci Technol. 2008;43:173-178.

10. McGee EJT, Diosady LL. Prevention of iron-polyphenol complex formation by chelation in black tea. LWT. 2018;89:756-762.

11. Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev. 1998;56(11):317-333.

12. D’Archivio M, Filesi C, Varì R, Scazzocchio B, Masella R. Bioavailability of the polyphenols: status and controversies. Int J Mol Sci. 2010;11(4):1321-1342.

13. Abdel-Shafy H, Mansour MSM. Polyphenols: properties, occurrence, content in food and potential effects. In: Phenolic Compounds – Biological Activity. InTech; 2017. p. 232-261.

14. Scalbert A, Williamson G. Dietary intake and bioavailability of polyphenols. J Nutr. 2000;130(8 Suppl):2073S-2085S.

15. Arfaoui L. Dietary plant polyphenols: effects of food processing on their content and bioavailability. Molecules. 2021;26(10):2959.

16. Jiménez-Monreal AM, García-Diz L, Martínez-Tomé M, Mariscal M, Murcia MA. Influence of cooking methods on antioxidant activity of vegetables. J Food Sci. 2009;74(3):H97-H103.

17. Nayak B, Liu RH, Tang J. Effect of processing on phenolic antioxidants of fruits, vegetables, and grains: a review. Crit Rev Food Sci Nutr. 2015;55(7):887-919.

18. Arts ICW, van de Putte B, Hollman PCH. Catechin contents of foods commonly consumed in The Netherlands. 1. Fruits, vegetables, staple foods, and processed foods. J Agric Food Chem. 2000;48(5):1746-1751.

19. Miglio C, Chiavaro E, Visconti A, Fogliano V, Pellegrini N. Effects of different cooking methods on nutritional and physicochemical characteristics of selected vegetables. J Agric Food Chem. 2008;56(1):139-147.

20. Koch W, Czop M, Nawrocka A, Wiącek D. Contribution of major groups of food products to the daily intake of selected elements: results from analytical determinations supported by chemometric analysis. Nutrients. 2020;12(11):3412.

21. Everette JD, Bryant QM, Green AM, Abbey YA, Wangila GW, Walker RB. Thorough study of reactivity of various compound classes towards the Folin-Ciocalteu reagent. J Agric Food Chem. 2010;58(14):8139-8144.

22. Koch W, Baj T, Kukula-Koch W, Marzec Z. Dietary intake of specific phenolic compounds and their effect on the antioxidant activity of daily food rations. Open Chem. 2015;13:869-876.

23. Grosso G, Stepaniak U, Topor-Mądry R, Szafraniec K, Pająk A. Estimated dietary intake and major food sources of polyphenols in the Polish arm of the HAPIEE study. Nutr. 2014;30(11-12):1398-1403.

24. Chun OK, Chung SJ, Song WO. Estimated dietary flavonoid intake and major food sources of U.S. adults. J Nutr. 2007;137(5):1244-1252.

25. Mullie P, Clarys P, Deriemaeker P, Hebbelinck M. Estimation of daily human intake of food flavonoids. Plant Foods Hum Nutr. 2007;62(3):93-98.

26. Koch W, Kukula-Koch W, Komsta Ł. Black tea samples origin discrimination using analytical investigations of secondary metabolites, antiradical scavenging activity and chemometric approach. Molecules. 2018;23(3):513.

27. Khan N, Mukhtar H. Tea polyphenols in promotion of human health. Nutrients. 2019;11(1):39.

28. Ovaskainen ML, Törrönen R, Koponen JM, Sinkko H, Hellström J,

Reinivuo H, et al. Dietary intake and major food sources of polyphenols in Finnish adults. J Nutr. 2008;138(3):562-566.

29. Wilczyńska A, Retel M. Oszacowanie pobrania związków fenolowych z dietą z uwzględnieniem udziału miodów pszczelich. Żywność Nauka Technologia Jakość. 2011;18(1):66-75.

30. Rejman K, Kowrygo B, Laskowski W. Evaluation of the structure of food consumption in Poland in the context of sustainable consumption. J Agribus Rural Dev. 2015;3(37):503-512.

31. Jąder K. Consumption of vegetables in Poland in different types of households. Roczn Nauk Stow Ekon Rol Agrobiz. 2015;17(3):144-150.

32. Gantner M, Stokowska B. Not only spices prolong the durability

of meat. Postepy Tech Przetw Spoż. 2015;1:86-90.

33. Pérez-Lamela C, Franco I, Falqué E. Impact of high-pressure processing on antioxidant activity during storage of fruits and fruit products: a review. Molecules. 2021;26(17):5265.

34. Saura-Calixto F, Goñi I. Antioxidant capacity of the Spanish Mediterranean diet. Food Chem. 2006;94:442-447.

35. Pulido R, Hernández-García M, Saura-Calixto F. Contribution of beverages to the intake of lipophilic and hydrophilic antioxidants in the Spanish diet. Eur J Clin Nutr. 2003;57(10):1275-1282.

36. de Vries JHM, Janssen PLTMK, Hollman PCH, van Staveren WA, Katan MB. Consumption of quercetin and kaempferol in free-living subjects eating a variety of diets. Cancer Lett. 1997;114(1):141-144.

37. Hassan FAM, Roushdy EM, Kishawy ATY, Zaglool AW, Tukur HA, Saadeldin IM. Growth performance, antioxidant capacity, lipid-related transcript expression and economics of broiler chickens fed different levels of rutin. Animals (Basel). 2019;9(1):7.

38. Li H, Gu Y, Jin R, He Q, Zhou Y. Effects of dietary rutin supplementation on intestinal morphology, antioxidant capacity, immunity, and microbiota of aged laying hens. Antioxidants (Basel). 2022;11(9):1843.

39. Boyle SP, Dobson VL, Duthie SJ, Hinselwood DC, Kyle JAM, Collins AR. Bioavailability and efficiency of rutin as an antioxidant: a human supplementation study. Eur J Clin Nutr. 2000;54(10):774-782.

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Published

2026-01-12

How to Cite

Tokarczyk, J., Czop, M., & Koch, W. (2026). Dietary intake of polyphenolic compoundsfrom major food groups: results of analytical determinations. Current Issues in Pharmacy and Medical Sciences, 38(4 (AOP). https://doi.org/10.12923/