Vol. 34 No. 1 (2021), Artykuły
Vol. 34 No. 1 (2021)

Antioxidant and antiproliferative activities of then-butanol extract of Centaurea maroccana Ball aerial parts

Artykuły
Published 2021-03-22
Imen Aissous
Laboratory of Biology and Environment, Faculty of Sciences of Nature and Life, University of Mentouri Constantine 1, Constantine, Algeria
Mouad Benrebai
Laboratory of Biology and Environment, Faculty of Sciences of Nature and Life, University of Mentouri Constantine 1, Constantine, Algeria
Ercan Cacan
Tokat Gaziosmanpasa University, Department of Molecular Biology and Genetics, Tokat, Turkey
Berkel Caglar
Tokat Gaziosmanpasa University, Department of Molecular Biology and Genetics, Tokat, Turkey
Ramazan Erenler
Tokat Gaziosmanpasa University, Faculty of Arts and Sciences, Department of Chemistry, Tokat, Turkey
Souad Ameddah
Laboratory of Biology and Environment, Faculty of Sciences of Nature and Life, University of Mentouri Constantine 1, Constantine, Algeria
Samir Benayache
Valorization of Natural Resources Unit, Bioactive Molecules and Physicochemical and Biological Analysis (VARENBIOMOL), University of Mentouri Constantine 1, Constantine, Algeria
Fadila Benayache
Valorization of Natural Resources Unit, Bioactive Molecules and Physicochemical and Biological Analysis (VARENBIOMOL), University of Mentouri Constantine 1, Constantine, Algeria
Chawki Bensouici
Biotechnology Research Center (CRBt), Algeria
https://orcid.org/0000-0003-4612-4642
PDF

Keywords

phenolic contents
antioxidant activity
anti-proliferative activity
Centaurea maroccana

Abstract

The aim of the present study is to evaluate the total phenolic contents, antioxidant and anti-proliferative activities of the n-BuOH extract of Centaurea maroccana (BECM). The total phenolic and flavonoid of the butanolic extract of the plant were assessed by using Folin-Ciocalteau and aluminium chloride colorimetric assays, respectively, and the quantitative estimation of total flavonoids and phenols revealed the richness of the extract in these compounds. Antioxidant activity was evaluated using standard lab colorimetric methods, while the anti-proliferative activity was evaluated using sulforhodamine B (SRB) assay against C6 (Rattus norvegicus brain glioma) and HT29 (Homo sapiens colorectal adenocarcinoma) cell lines. The n-butanolic extract of Centaurea maroccana showed a strong antioxidant activity through DPPH, ABTS•+, DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. Furthermore, the anti-proliferative activity against C6 and HT29 of Centaurea maroccana exhibited a high effect by the decrease in viability of both cancer cell lines. Our results suggest a possible use of Centaurea maroccana as a source of natural antioxidant and chemo-preventive agents against cancer.

PDF

References

1. Adebiyi OE, Olayemi FO, Ning-Hua T, Guang-Zhi Z. In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni-Suef Univ. J Basic Appl Sci. 2017;6(1):10-4.

2. Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food Chem Toxicol. 2013;55:290-6.

3. Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006;97(4):654-60.

4. Noreen H, Semmar N, Farman M, McCullagh JSO. Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus. Asian Pac J Trop Med. 2017;10(8):792-801.

5. Das N, Islam ME, Jahan N, Islam MS, Khan A, Islam MR, Parvin M S. Antioxidant activities of ethanol extracts and fractions of Crescentia cujete leaves and stem bark and the involvement of phenolic compounds. BMC Complement Altern Med. 2014;14(45).

6. Ayad R, Ababsa ZA, Belfadel FZ, Akkal S, León F, Brouard I, Medjroubi K. Phytochemical and biological activity of Algerian Centaurea melitensis. Int J Med Arom Plants. 2012;2:151-4.

7. Louaar S, Zellagui A, Gherraf N, Medjroubi K, Derbre S, Seguin E, et al. Antiradical activity of flavonoids from the Algerian Native Plant: Centaurea microcarpa Coss. et Dur. J Biol Act Prod Nat.2014;4(3):249-53.

8. Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Screening for in vitro antioxidant properties and fatty acid profiles of five Centaurea L. species from Turkey flora. Food Chem Toxicol. 2011;49(11):2914-20.

9. Khammar A, Djeddi S. Pharmacological and biological properties of some Centaurea species. Eur J Sci Res. 2012;84:398-416.

10. Zengin G, Cakmak YS, Guler GO, Aktumsek A. In vitro antioxidant capacities and fatty acid compositions of three Centaurea species collected from Central Anatolia region of Turkey. Food Chem Toxicol. 2010;48(10):2638-41.

11. Erel SB, Karaalp C, Bedir E, Kaehlig H, Glasl S, Khan S, Krenn L. Secondary metabolites of Centaurea calolepis and evaluation of cnicin for anti-inflammatory, antioxidant, and cytotoxic activities. Pharm Biol. 2011;49(8):840-9.

12. Güven K, Çelik S, Uysal I. Antimicrobial activity of Centaurea species. Pharm Biol. 2005;43(1):67-71.

13. Koca U, Özçelik B. Antiviral, antibacterial, and antifungal activities of Centaurea tchihatcheffii extracts. Turk J Pharm Sci. 2009;6(2):125-34.

14. Erol-Dayi Ö, Pekmez M, Bona M, Aras-Perk A, Arda N. Total phenolic contents, antioxidant activities cytotoxicity of three Centaurea species: C. calcitrapa subsp. calcitrapa, C. ptosimopappa and C. spicata. Free Rad Antiox. 2011;1(2):31-6.

15. Mezache N, Bendjeddou D, Satta D, Mekkiou R, Benayache S, and Benayache F. Secondary metabolites from Centaurea lippii. Chem Nat Compd. 2010;46:801-2.

16. Grafakou ME, Djeddi S, Hamel T, Skaltsa H. Secondary metabolites from the aerial parts of Centaurea papposa (Coss.) Greuter. Biochem Syst Ecol. 2018;76:15-22.

17. Bicha S, Bentamene A, Benaissa O, Benayache S, Garcia VP, Leon F, et al. Flavonoid aglycones from Centaurea maroccana. Chem Nat Compd. 2011;47:105-6.

18. Quezel P, Santa S. Nouvelle Flore de l’Algérie et des régions désertiques méridionales. Paris: Centre National de La Recherche Scientifique (CNRS) ;1963 :1016-32.

19. Muller L, Gnoyke S, Popken AM, Bohm V. Antioxidant capacity and related parameters of different fruit formulations. LWT Food Sci Technol. 2010;43(6):992-9.

20. Topçu G, Ay A, Bilici A, Sarıkürkcü C, Öztürk M, Ulubelen A. A new flavone from antioxidant extracts of Pistacia terebinthus. Food Chem. 2007;103(3):816-22.

21. Blois MS. Antioxidants determination by the use of a stable free radical. Nature. 1958;26:1199-200.

22. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26 (9-10):1231-7.

23. Kunchandy E, Rao MNA. Oxygen radical scavenging activity of curcumin. Int J Pharm. 1990;58(3):237-40.

24. Bouratoua A, Khalfallah A, Bensouici C, Kabouche Z, AlabdulMagi A, Harakat D, et al. Chemical composition and antioxidant activity of aerial parts of Ferula longipes Coss. ex Bonnier and Maury. Nat Prod Res. 2017;32(16):1873-80.

25. Marco GJ. A rapid method for evaluation of antioxidants. J Am Oil Chem Soc. 1968;45:594-8.

26. Apak R, Güçlü K, Ozyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agric Food Chem. 2004;52(26):7970-81.

27. Vichai V, Kirtikara K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat. Protoc. 2006;1(3):1112-6.

28. Tel-Çayan G, Deveci E, Çayan F, Duru ME. Comparative assessment of phytochemical composition, antioxidant and anticholinesterase activities of two Basidiomycota Truffle Fungi from Turkey. Marmara Pharm J. 2018;22 (1):59-65.

29. Tel G, Apaydın M, Duru ME, Öztürk M. Antioxidant and cholinesterase inhibition activities of three Tricholoma species with total phenolic and flavonoid contents: The edible mushrooms from anatolia. Food Anal Methods. 2011; 5(3):495-504.

30. Phang CW, Sri-Nurestri AM, Halijah I. Antioxidant potential, cytotoxic activity and total phenolic content of Alpinia pahangensis rhizomes. BMC Complement Altern Med. 2013;13(1).

31. Nishaa S, Vshnupriya M, Gopalakrishnan VK. Qualitative assessment of ethanolic extract of Maranta arundinacea L.rhizome using HPLC. Int Res J Pharm.2013;4(2):76-83.

32. Kalim MD, Bhattacharyya D, Banerjee A, Chattopadhyay S. Oxidative DNA damage preventive activity and antioxidant potential of plants used in Unani system of medicine. BMC Complement Altern Med. 2010;10(1).

33. Krishnamoorthy M, Sasikumar J, Shamna R, Pandiarajan C, Sofia P, Nagarajan B. Antioxidant activities of bark extract from mangroves, Bruguiera cylindrica (L.) Blume and Ceriops decandra Perr. Indian J Pharmacol.2011;43(5):557.

34. Ansari AQ, Syed Abrar A, Waheed MA, Sayyed Juned A. Extraction and determination of antioxidant activity of Withania somnifera Dunal. Eur J Exp Biol. 2013;3(5):502-7.

35. Chandrashekhar HR, Venkatesh P, Arumugam M, Vijayan P. Estimation of total phenols with special emphasis to antioxidant potentials of few Hypericum species. Pharmacologyonline. 2009;1:680-7.

36. Lalhminghlui K, Jagetia GC. Evaluation of the free-radical scavenging and antioxidant activities of Chilauni, Schima wallichii Korth in vitro. Future Sci OA. 2018;4(2).

37. Kumar RS, Rajkapoor B, Perumal P. Antioxidant activities of Indigofera cassioides Rottl. Ex. DC. using various in vitro assay models. Asian Pac J Trop Biomed. 2012; 2(4):256-61.

38. Jamuna S, Paulsamy S and Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). J Appl Pharm Sci. 2012; 02(07):149-54.

39. Saha M, Hasan S, Akter R, Hossain M, Alam M, Alam M, Mazumder M. In vitro free radical scavenging activity of methanol extract of the leaves of Mimusops elengi Linn. Bangladesh J Vet Med. 2008;6(2):197-202.

40. Öztürk M, Duru ME, Kivrak Ş, Mercan-Doğan N, Türkoglu A, Özler MA. In vitro antioxidant, anticholinesterase and antimicrobial activity studies on three Agaricus species with fatty acid compositions and iron contents: A comparative study on the three most edible mushrooms. Food Chem Toxicol. 2011;49(6):1353-60.

41. Laggoune S, Öztürk M, Erol E, Duru ME, Abaza I, Kabouche A, Kabouche Z. Chemical composition, antioxidant and antibacterial activities of the essential oil of Mentha spicata L. from Algeria. J Mater Environ Sci. 2016;7:4205-13.

42. Tusevski O, Kostovska A, Iloska A, Trajkovska L, Simic S. Phenolic production and antioxidant properties of some Macedonian medicinal plants. Cent Eur J Biol. 2014;9(9):888-900.

43. Vindhya K, Leelavathi S. Evaluation of antioxidant properties and total phenolic content of Gardenia gummifera Linn. Int J Pharm Sci Rev Res. 2015;32 (1):255-61.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.

Copyright (c) 2021 Authors

Downloads

Download data is not yet available.