Keywords
sodium hypochlorite
erythrocytes
lipid peroxidation – LP
sodium hypochlorite – SH
thiobarbituric acid – TBA
Abstract
We studied the simultaneous influence of histamine and sodium hypochlorite (SH) on lipid peroxidation processes, as well as the level of structural changes in membranes (via the content of sialic acid) in rat erythrocytes. We established that histamine affects lipid peroxidation processes with the formation of lipid hydroperoxides, damages proteins and reduces the content of sialic acids, which leads to changes in the surface charge of red blood cells. However, the simultaneous action of histamine and low SH concentration has a positive effect in that it corrects the pro-oxidant state of erythrocytes. Hence, the content of lipid hydroperoxides, TBA-active products, carbonyl groups of proteins and sialic acids were mainly reduced after the simultaneous action of histamine and SH at all studied concentrations during the rehabilitation period.References
1. Vitushinska M, Matiytsiv N, Chernik Ya. Sensitivity to the oxidative stress conditions lifespan and neurodegenerative changes in the brain structure of Drosophila melanogaster superoxiddismutase mutants. Visn Lviv Univ Biol Ser. 2013;62:108-16.
2. Kanyuka OP, Filyak YeZ, Sybirna NO. Peculiarities of structural and functional organization of erythrocyte membranes in mice of wild-type and mice with PTTG gene knockout. Stud Biol. 2011;5(1):97-104.
3. Girina OM, Karlova O, Bruzgina TS. Evaluation of lipid profiles of erythrocytes in patients with metabolic syndrome. Med Chem. 2007;9(1):72-5.
4. Apikhtina OL. Investigation of the toxic effect of heavy metals on the blood erythrocyte model in vitro. Mod Probl Tox. 2011;1-2:65-9.
5. Komarenko VI, Terekhov AA, Vorobyova AP. Investigation of the role of H1-receptor responses in rat liver portal vessels to histamine. Cherk Nat Univ B Khmelnitsky Biol Sci Series. 2008;128: 54-8.
6. Oleksiuk NP, Yanovych VG. The activity of pro- and antioxidant systems in the liver of freshwater fishes in different seasons. Ukr Biochem J. 2010;82(3):41-8.
7. Timirbulatov RR, Seleznev ЕI. Method of increase intensity of free-radical oxidization of lipid components of blood and his diagnostic value. Lab Bus. 1981;4:209-11.
8. Morozenko DV, Leontieva FS. Research methods markers of connective tissue metabolism in modern clinical and experimental medicine. Young Scien. 2016;2(29):168-72.
9. Meschyshyn IF, Poloviy VP. Mechanism of oxidative modification of proteins. Buk Med Herald. 1999;3(1):196-205.
10. Kawai Y, Nuka E. Abundance of DNA adducts of 4-oxo-2-alkenals, lipid peroxidation-derived highly reactivgenotoxins. J Clin Biochem Nutr. 2018;62(1):3-10.
11. Michael M, Brent R. Lipid peroxidation in cell death. Biochem Biophys Res Commun. 2017;482(3):419-25.
12. Iskusnyh AYu, Basharina OV, Artyukhov VG, Alabovsky AA. The influence of histamine on the functional properties of neutrophils and the intensity of the process of peroxide oxidation of lipids in the blood of donors. Vest VSU Ser. Chem Biol Phar. 2008;1:93-6.
13. Shaiko LM, Bokut SB. Workshop on medical and biological physics. Section “Biological Physics”: Methods of Biophysical Research. Minsk: A.D. Sakharov Moscow State Polytechnic Institute; 2011.
14. Lin YW. Structure and function of heme proteins regulated by diverse post-translational modifications. Arch Biochem Biophys. 2018;641:1-30.
15. Miyamoto S, Di Mascio P. Lipid hydroperoxides as a source of singlet molecular oxygen. Subcell Biochem. 2014;77:3-20.
16. Andriichuk AV, Tkachenko HM, Kurhaluk NM, Tkachova IV. Dynamics of hematological parameters and oxidative stress markers in Ukrainian warmblood horses under exercise influence The J of V.N. Karazin Kharkiv National Univ. Ser Biol. 2013;17(1056):148-60.
17. Anu P, Padler-Karavani V. Evolution of sialic acids: Implications in xenotransplant biology. Wiley Xenotransplant. 2018;12424:1-11.
18. Osoba IA. The biological role of lipid peroxidation in ensuring the functioning of fish organism. Fish Sci Ukr. 2013;1:87-96.
19. Radi R. Oxygen radicals, nitric oxide, and peroxynitrite: Redox pathways in molecular medicine. Proc Natl Acad Sci (USA) 2018;115(23): 5839-48.
20. Popova E, Koshchiy I. Role of lipids in adaptation of fish to ecological stress. Fish Sci Ukr. 2007;1:49-56.
21. Halverson GR, Reid ME, Sutherland J, Rhodes M. Evaluation and comparison of three human monoclonal anti-S, two human polyclonal anti-S, and one murine anti-GPB. Immunohematol. 1999;15(4):163-6.
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