Keywords
hybrid tbf gene
Sanger sequencing
protein design
TBF recombinant protein
immunodominant epitopes
Vibrio choleraе
Abstract
We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also calculated the nucleatic sequence of the tbf gene. The obtained results have both scientific and practical significance.
References
1. Kiaie S, Abtahi H, Mosayebi G, Alikhani M, Pakzad I. Recombinant toxin-coregulated pilus A (TcpA) as a candidate subunit cholera vaccine. Iran J Microbiol. 2014;6:68-73.
2. Megli CJ, Yuen AS, Kolappan S, Richardson MR, Dharmasena MN. Crystal structure of the Vibrio cholerae colonization factor TcpF and identification of a functional immunogenic site. Mol Biol. 2011;409:146-58.
3. Liljeqvist S. Surface display of the cholera toxin B subunit on Staphylococcus xylosus and Staphylococcus carnosus. Appl Environ Microbiol. 1997;63:2481-8.
4. Sanchez J, Johansson S, Löwenadler B, Svennerholm A, Holmgren J. Recombinant cholera toxin B subunit and gene fusion proteins for oral vaccination. Research in Microbiology. 1990;141:971-9.
5. Praetor A, Jones RM, Wong WL, Hunziker W. Membrane-anchored human FcRn can oligomerize in the absence of IgG. J. Mol. Biol. 2002;321:277-84.
6. Schroeder HW, Cavacini L. Structure and function of immuno-globulins. J Allergy Clin Immunol. 2010:125(2,Suppl 2):S41-52. DOI
7. Raghavan M, Bjorkman PJ. Fc receptors and their interactions with immunoglobulins. Annu Rev Cell Dev Biol. 1996;12:181-220.
8. Reed SM, Bayly WM, Sellon DC. Equine internal medicine (4 edition). St. Louis, Missouri: Elsevier;2018:1566.
9. Addgene: pET28a(+) www.addgene.org/20378/.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.
Copyright (c) 2019 Autors