Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Authors

  • Yulia Bazarnova Higher School of Biotechnology and Food Technology, Peter the Great Saint Petersburg State Polytechnical University Author https://orcid.org/0000-0001-9275-7913
  • Tatiana Bolotnikova Higher School of Biotechnology and Food Technology, Peter the Great Saint Petersburg State Polytechnical University Author
  • Ekaterina Aronova Higher School of Biotechnology and Food Technology, Peter the Great Saint Petersburg State Polytechnical University Author

DOI:

https://doi.org/10.2478/cipms-2019-0024

Keywords:

candidate cholera vaccine, hybrid tbf gene, Sanger sequencing, protein design, TBF recombinant protein, immunodominant epitopes, Vibrio choleraе

Abstract

We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also calculated the nucleatic sequence of the tbf gene. The obtained results have both scientific and practical significance.

References

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Published

2019-09-28

Issue

Vol. 32 No. 3 (2019): Current Issues of Pharmacy and Medical Sciences

Section

Artykuły

License

Copyright (c) 2019 Autors

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.

How to Cite

Bazarnova, Y., Bolotnikova, T., & Aronova, E. (2019). Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains. Current Issues in Pharmacy and Medical Sciences, 32(3), 130-133. https://doi.org/10.2478/cipms-2019-0024