Ultrastructural changes in murine liver following HSV infection and stroke

Authors

  • Natalia Motorna O.O. Bogomolets National Medical University, Department of Histology and Embryology, T. Shevchenko Blvd 13, 01601 Kyiv, Ukraine Author
  • Svetlana Rybalko Gromashevsky L.V. Institute of Epidemiology and Infection Diseases of NAMS of Ukraine, Kyiv, Ukraine Author
  • Tatyana Kvitnitskaya-Ryzhova State Institution “D.F. Chebotarev Institute of Gerontology” of NAMS of Ukraine, Kyiv, Ukraine Author
  • Daria Starosyla Gromashevsky L.V. Institute of Epidemiology and Infection Diseases of NAMS of Ukraine, Kyiv, Ukraine Author
  • Iryna Strokina O.O. Bogomolets National Medical University, Department of Histology and Embryology, T. Shevchenko Blvd 13, 01601 Kyiv, Ukraine Author
  • Rostyslav Kaminsky O.O. Bogomolets National Medical University, Department of Histology and Embryology, T. Shevchenko Blvd 13, 01601 Kyiv, Ukraine Author
  • Sergey Savosko O.O. Bogomolets National Medical University, Department of Histology and Embryology, T. Shevchenko Blvd 13, 01601 Kyiv, Ukraine Author
  • Liudmyla Sokurenko O.O. Bogomolets National Medical University, Department of Histology and Embryology, T. Shevchenko Blvd 13, 01601 Kyiv, Ukraine Author
  • Yuri Chaikovsky O.O. Bogomolets National Medical University, Department of Histology and Embryology, T. Shevchenko Blvd 13, 01601 Kyiv, Ukraine Author

DOI:

https://doi.org/10.1515/cipms-2018-0002

Keywords:

liver, ultrastructure, herpes simplex virus type I, stroke

Abstract

The study of herpetic infection is a topical problem. Although the peculiarities and consequences of acute HSV-I infection in the brain are quite well-studied, little is known about the damage to other organs which are not a source of latent HSV-I infection, the liver in particular. The current study is aimed at determining the ultrastructural changes in murine liver following HSV infection and stroke. Liver samples obtained from four groups of animals were studied: 1) intact mice; 2) mice with stroke; 3) mice infected with HSV-I; 4) mice aflicted with HSV-I and subsequently simulated stroke. The study showed the reproduction of the virus in hepatic endotheliocytes, although no virions were detected in the hepatocytes. Therefore, the described changes were considered the consequences of the infectious process. Pathological changes of hepatocytes consisted of deformation and fragmentation of the nuclei, as well as accumulation of osmiophilic granules, lysosomes and lamellary bodies. Latent HSV-I infection may reactivate in liver after the stroke, potentially causing the complications of the underlying disease.

References

1. Xu F, Schillinger JA, Sternberg MR, et al: Seroprevalence and coinfection with herpes simplex virus type 1 and type 2 in the United States, 1988-1994. J Infect Dis. 2002;185:1019-24. Google Scholar

2. Yao HW, Ling P, Tung YY, Hsu SM and Chen SH: In vivo reactivation of latent herpes simplex virus 1 in mice can occur in the brain before occurring in the trigeminal ganglion. J Virol. 2014;88: 11264-70. Google Scholar

3. Hara Y, Ishii N, Sakai K, et al. Herpes simplex encephalitis initially presented with parietal cortex lesions mimicking acute ischemic stroke: A case report. Rinsho Shinkeigaku 2016;56:104-7. Google Scholar

4. Pires de Mello CP, Bloom DC and Paixão IC: Herpes simplex virus type-1: replication, latency, reactivation and its antiviral targets. Antivir Ther. 2016;21:277-86. Google Scholar

5. Norvell JP, Blei AT, Jovanovic BD and Levitsky J: Herpes simplex virus hepatitis: an analysis of the published literature and institutional cases. Liver Transpl. 2007;13:1428-34. Google Scholar

6. Gumenyuk A, Motorna N, Rybalko S, Savosko S, Sokurenko L, Starosyla D, Porva Yu, Chaikovsky Yu. Development of herpetic infection associated with stroke and its correction with acyclovir. Curr Issues Pharm Med Sci. 2017;30(1):20-3. Google Scholar

7. Gumenyuk AV, Motorna NV, Rybalko SL, Savosko SI, Sokurenko LM and Chaikovsky YuB: Mutual influence of herpes virus infection activation and cerebral circulation impairment on the state of brain cells. Biopolym Cell 2016;32:126-30. Google Scholar

8. Navaneethan U, Lancaster E, Venkatesh PG, Wang J and Neff GW: Herpes simplex Virus hepatitis – It’s high time We Consider Empiric treatment. J Gastrointestin Liver Dis. 2011;20:93-6. Google Scholar

9. Ichai P, Roque Afonso AM, Sebagh M, et al: Herpes simplex virus-associated acute liver failure: a difficult diagnosis with a poor prognosis. Liver Transpl. 2005;11:1550-5. Google Scholar

10. Motorna N, Rybalko SL, Sokurenko LM, Starosyla DB, Savosko SI and Chaikovsky YuB. Patterns of herpetic infection reactivation in the liver. Microbiology Journal 2017;2:70-9. Google Scholar

11. Muscari A, Collini A, Fabbri E, Giovagnoli M, Napoli C, Rossi V, Vizioli L, Bonfiglioli A, Magalotti D, Puddu GM, and Zoli M. Changes of liver enzymes and bilirubin during ischemic stroke: mechanisms and possible significance. BMC Neurol. 2014;14:122. Google Scholar

12. Fujii Y, Takeuchi S, Tanaka R, et al: Liver dysfunction in spontaneous intracerebral hemorrhage. Neurosurgery 1994;35(4):592-6. Google Scholar

13. Hoya K, Tanaka Y, Uchida T, Takano I, Nagaishi M, Kowata K and Hyodo A. Intracerebral hemorrhage in patients with chronic liver disease. Neurol Med Chir (Tokyo) 2012;52(4):181-5. Google Scholar

Downloads

Published

2018-04-11

Issue

Vol. 31 No. 1 (2018): Current Issues of Pharmacy and Medical Sciences

Section

Artykuły

License

Copyright (c) 2018 Authors

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.