Vol. 27 No. 4 (2014), Artykuły
Vol. 27 No. 4 (2014)

The effect of thyroxin on hepatic redox equilibrium and lipid metabolism in rats treated with doxorubicin

Artykuły
Published 2015-03-03
Bartosz Czuba
Independent Medical Biology Unit, Medical University of Lublin, Jaczewskiego 8, 20-950 Lublin, Poland
Magdalena Fituch
Independent Medical Biology Unit, Medical University of Lublin, Jaczewskiego 8, 20-950 Lublin, Poland
Slawomir Mandziuk
Department of Pneumology, Oncology and Alergology, Medical University of Lublin, Jaczewskiego 8, 20-090 Lublin, Poland
Barbara Jodlowska-Jedrych
Department of Histology and Embryology, Medical University of Lublin, Radziwillowska 11, 20-080 Lublin, Poland
Wlodzimierz Matysiak
Department of Histology and Embryology, Medical University of Lublin, Radziwillowska 11, 20-080 Lublin, Poland
Justyna Halasa
Department of Anatomy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland
Franciszek Burdan
Department of Anatomy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland / St. John’s Cancer Center, Jaczewskiego 7, 20-090 Lublin, Poland
Agnieszka Korga
Independent Medical Biology Unit, Medical University of Lublin, Jaczewskiego 8, 20-950 Lublin, Poland
Magdalena Iwan
Independent Medical Biology Unit, Medical University of Lublin, Jaczewskiego 8, 20-950 Lublin, Poland
Iwona Luszczewska-Sierakowska
Department of Animal Anatomy and Histology, University of Life Sciences, Akademicka 12, 20-950, Lublin, Poland
Jarosław Dudka
Independent Medical Biology Unit, Medical University of Lublin, Jaczewskiego 8, 20-950 Lublin, Poland / Chair and Department of Toxicology, Medical University of Lublin, Chodzki 8, 20-093 Lublin, Poland
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Keywords

doxorubicin
thyroxin
liver
redox equilibrium
lipid metabolism

Abstract

The main side effects of the administration of doxorubicin, a widely used anticancer drug, is the generation of a reactive oxygen species (ROS) in normal cells. As a result, redox disorders and secondary oxidative stress are developed. Doxorubicin ROS generation is attributed to enzymes that are produced abundantly in hepatocytes. Oxidative stress has been a well-known risk factor of doxorubicin-related toxicity. However, in addition, according to the data collected in the last decade, changes in thyroxin status can propagate ROS generation, and, thus, initiate the doxorubicin hepatic effect. Moreover, both compounds have an impact on the cell metabolism. The aim of the study was to verify the thesis that thyroxin can modulate the effect of doxorubicin with regard to redox status and lipid metabolism disorders. In our work, we determined the ratio of NADP+/ NADPH and NAD+/NADH in liver homogenates, blood ketone bodies and triglycerides in the liver and blood in rats treated with doxorubicin and thyroxin. Our results indicate that thyroxin has an insignificant effect on NAD+/NADH, NADP+/NADPH ratios and on hepatic and blood triglycerides. Moreover, thyroxin administration normalized the level of blood ketone bodies that was disturbed by doxorubicin.

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