Vol. 27 No. 2 (2014), Artykuły
Vol. 27 No. 2 (2014)

The influence of proteasome inhibitor on the expression of cardiomyocytes damage markers after incubation with doxorubicin

Artykuły
Published 2014-11-25
Sylwia Tereszkiewicz
Independent Medical Biology Unit, Medical University of Lublin, Poland
Wlodzimierz Matysiak
Department of Histology and Embryology, Medical University of Lublin, Poland
Slawomir Mandziuk
Department of Pneumology Oncology and Allergology, Medical University of Lublin, Poland
Agnieszka Korga
Independent Medical Biology Unit, Medical University of Lublin, Poland
Malgorzata Oleksiejuk
IMMUNIQ, Beata Solon-Gogol, Sasiedzka 1, 44-240 Zory, Poland
Marcin Hejna
Regional Dental Clinic Independent Public Health Care, Lubartowska 58, Lublin, Poland
Agnieszka Korobowicz-Markiewicz
Department of Pediatric Pulmonology and Rheumatology, Medical University of Lublin, Poland
Magdalena Iwan
Independent Medical Biology Unit, Medical University of Lublin, Poland
Jaroslaw Dudka
Independent Medical Biology Unit, Medical University of Lublin, Poland
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Keywords

doxorubicin
proteasom
BNP
H-FABP

Abstract

The aim of the study was to verify the thesis that the cardiotoxic effects of doxorubicin are connected with activation of the ubiquitin - proteasome pathway followed by protein degradation. The expression of myocardial damage markers - fatty acid binding protein (H-FABP) and brain natriuretic peptide (BNP) was evaluated in rat fetal cardiomyocytes simultaneously treated with doxorubicin and the proteasome inhibitor - bortezomib. The level of H-FABP and BNP protein under the influence of doxorubicin was decreased below the detection threshold with unchanged (H-FABP) or elevated (BNP) mRNA expression level. Against the expectations, the inhibitor of proteasome did not abolish this effect. The observed abnormal expression of BNP and H-FABP protein after doxorubicin treatment makes their diagnostic significance in anthracycline cardiotoxicity questionable.

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