Vol. 22 No. 4 (2009), Artykuły
Vol. 22 No. 4 (2009)

Determination of free and bound phenolic acids in leaves and fruitsof Pyracantha coccinea (L.) Roem. (Rosaceae)

Artykuły
Published 2009-12-30
Anna Sokołowska-Krzaczek
Department of Pharmaceutical Botany, Medical University of Lublin
Katarzyna Szewczyk
Department of Pharmaceutical Botany, Medical University of Lublin
Zofia Bawół
Department of Pharmaceutical Botany, Medical University of Lublin

Abstract

The analysis of the fractions of free and liberated by hydrolysis phenolic acids in the fruits and the leaves of Pyracantha coccinea was conducted by TLC and RP-HPLC methods. In the fruits eight and in the leaves thirteen phenolic acids were identified. By means of the RP-HPLC method the content of major acids was estimated, which ranges from 0.002 up to 17.38 mg/100 g of the dry material. Protocatechuic acid is a remarkably dominating constituent of both plant materials.

PDF

References

1. Bilia A.R., Catalano S.: Flavonoids from Pyracantha coccinea roots. Phytochem. 33(6), 1449, 1993.

2. Bilia A.R., Flamini G.: New constituents from Pyracantha coccinea leaves. J. Nat. Prod. 56(6), 984, 1993.

3. Bilia A.R., Morelli I.: Two glucosides from Pyracantha coccinea roots: a new lignin and a new chalcone. Tetrahedron, 50(17), 5181, 1994.

4. Borkowski B.: Fenolokwasy i ich estry. I. Herba Pol., 39, 71, 1993.

5. Bugała W.: Drzewa i krzewy. PWRiL, Warszawa 2000.

6. Chojnowska E.: Ozdobne krzewy i pnącza. Zapolex sp. z o.o., Warszawa 2000.

7. Dzido T. H.: The Horizontal DS.-Chamber and its Modifications. J. Planar Chromatogr., 6, 78, 1993.

8. Farmakopea Polska VI, Warszawa 2002.

9. Fico G., Bilia A. R.: Flavonoid distribution in Pyracantha coccinea plants at different growth phases. Biochem. Syst. Ecol. 28(7), 673, 2000.

10. Jerzmanowska Z.: Substancje roślinne. Metody wyodrębniania. PWN. 1967, Warszawa.

11. Krzaczek T.: Fenolokwasy w niektórych surowcach garbnikowych z rodziny Rosaceae. Farm. Pol. 40(8), 475, 1984.

12. Liskens H.T. Papierchromatographie In der Botanik. Springer Verlag, Berlin-Goettingen-Heidelberg 1959.

13. Negwer M.: Organic-chmial drugs an their synonys. Academe-Verlag, Berlin 1978.

14. Podbielkowski Z.: Słownik roślin użytkowych. PWRiL, Warszawa 1985.

15. Schmidtlein H., Herrmann K.: Quantitative analysis for phenolic acids by thin layer chromatography. J. Chromatogr. 115, 123, 1975.

16. Smolarz H. D., Waksmundzka-Hajnos M.: Two-dimensional TLC of phenolic acids on cellulose. J. Planar Chromatogr., 6, 278, 1993.

17. Świątek L., Dombrowicz E.: Kwasy fenolowe w surowcach goryczowych. Cz. I. Badanie ziela piołunu i korzenia goryczki. Farm. Pol. 40, 729, 1984.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.

Copyright (c) 2009 Authors

Downloads

Download data is not yet available.