Vol. 22 No. 4 (2009), Artykuły
Vol. 22 No. 4 (2009)

Nanobiomodification of vascular suture with vascular endothelialgrowth factor (VEGF) – in vivo preliminary study

Artykuły
Published 2009-12-30
Anna Belcarz
Chair and Department of Biochemistry, Medical University of Lublin
Stanisław Przywara
Department of Vascular Surgery and Angiology, Medical University of Lublin
Jacek Wroński
Department of Vascular Surgery and Angiology, Medical University of Lublin
Grażyna Ginalska
Chair and Department of Biochemistry, Medical University of Lublin
Justyna Szumiło
Department of Clinical Patomorphology, Medical University of Lublin
Elżbieta Korobowicz
Department of Clinical Patomorphology, Medical University of Lublin

Abstract

A method of quite stable binding of Vascular Endothelial Growth Factor (VEGF) to vascular suture has been developed. The effect of nanobiomodified suture on the healing process of sutured artery wall was evaluated in vivo in rabbit model, using histopathological, immunohistochemical and transmission electron microscopy methods. Results of transmission electron microscopy showed beneficial effects of artificial VEGF-coated sutures on the healing process. The presented VEGFcoated sutures improved the healing process and biomodified coatings did not produce histological appearance of biological toxicity or unusual tissue reactions.

PDF

References

1. Barker S.G., Talber t A., Cottam S. et al.: Arterial intimal hyperplasia after occlusion of the adventitial vasa vasorum in the pig. Arterioscler. Thromb., 13(1), 70, 1993.

2. Bates D.O., Harper S. J.: Regulation of vascular permeability by vascular endothelial growth factors. Vascul. Pharmacol., 39 (4-5), 225, 2002.

3. Belcarz A., Ginalska G., Zalewska J. et al.: Covalent coating of hydroxyapatite by keratin stabilizes gentamicin release. J. Biomed. Mater. Res. Part B. Applied Biomaterials, 89B, 102, 2009.

4. Byrne A.M., Bouchier-Hayes D. J., Harmey J. H.: Angiogenic and cell survival functions of vascular endothelial growth factor (VEGF). J. Cell. Mol. Med., 9 (4), 777, 2005.

5. Drake C. J., Lit t le C. D.: Exogenous vascular endothelial growth factor induces malformed and hyperfused vassels during embryonic neovascularization. Proc. Natl. Acad. Sci. USA, 92, 7657, 1995.

6. Ferrara N.: The role of VEGF in the regulation of physiological and pathological angiogenesis. EXS, 94, 209, 2005.

7. Ginalska G., Osińska M., Uryniak A. et al.: Antibacterial activity of gentamicin-bonded gelatin-sealed polyethylene terephthalate vascular prostheses. Eur. J. Vasc. Endovasc. Surg., 29, 419, 2005.

8. Hamdan A. D., Aiello L. P., Misare B. D. et al.: Vascular endothelial growth factor expression in canine peripheral vein bypass grafts. J. Vasc. Surg., 26 (1), 79, 1997.

9. Infanger M., Shakibaei M., Kossmehl P. et al.: Intraluminal application of vascular endothelial growth factor enhances healing of microvascular anastomosis in a rat model. J. Vasc. Res., 42 (3), 202, 2005.

10. Lindner V., Reidy M. A.: Expression of VEGF receptors in arteries after endothelial injury and lack of increased endothelial regrowth in response to VEGF. Arterioscler. Thromb. Vasc. Biol., 16 (11), 1399, 1996.

11. Petersen W., Pufe T., Stärke Ch. et al.: The effect of locally applied vascular endothelial growth factor an meniscus healing: gross and histological findings., Arch. Orthop. Trauma. Surg., 127, 235, 2007.

12. Seipelt R.G., Backer C.L., Mavroudis C. et al.: Topical VEGF enhances healing of thoracic aortic anastomosis for coarctation in a rabbit model. Circulation, 108 Suppl. 1, II 150, 2003.

13. Zachary I.: Signaling mechanisms mediating vascular protective actions of vascular endothelial growth factor Am. J. Physiol. Cell. Physiol., 280 (6), C1375, 2001.

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.

Copyright (c) 2009 Authors

Downloads

Download data is not yet available.