Differentiation by RAPD-PCR of Candida albicans isolated from upper respiratory tract in elderly people from care center

Authors

DOI:

https://doi.org/10.12923/

Keywords:

differentiation, RAPD-PCR, Candida albicans, upper respiratory tract

Abstract

Elderly people, aged 65 years old or older, are predisposed to fungal infections caused especially by Candida spp., mainly Candida albicans. Among them the most common are respiratory tract candidiasis. The aim of this study was to estimate the genetic diversity by RAPD-PCR of C. albicans isolated from the upper respiratory tract in elderly people from a  care center in order to assess spreading of these strains among people from close population. Among 75 strains, 46 genotypes were defined. 11 clusters comprising 40 (53.33%) isolates at the value of similarity coefficient ≥ 80% were formed. The remaining 35 (46.67%) isolates represented individual genotypes.

 

 

References

1. Chong P.P., Lee Y.L., Tan B.C.: Genetic relatedness of Candida strains isolated from women with vaginal candidiasis in Malaysia. J. Med. Microbiol., 52, 657, 2003.

2. Costa F., Manaia C.M., Figueiral M.H. et al.: Genotypic analysis of Candida albicans isolates obtained from removable prosthesis wearers. Lett. Appl. Microbiol., 46, 445, 2008.

3. Krawczyk B., Leibner-Ciszak J., Mielech A. et al.: PCR melting profile (PCR MP) – a new tool for differentiation of Candida albicans strains. BMC Infect. Dis., 9, 177, 2009.

4. Lee W., Low B.K., Samaranayake L.P. et al.: Genotypic variation of Candida albicans during orthodontic therapy. Front. Biosci., 1, 3814, 2008.

5. Pinto P.M., Resende M.A., Koga-Ito C.Y. et al.: Genetic variability analysis among clinical Candida spp. isolates using random amplified polymorphic DNA. Mem. Inst. Oswaldo Cruz, 99, 147, 2004.

6. Pires-Gonçalves R.H. Miranda E.T., Baeza L.C. et al.: Genetic relatedness of commensal strains of Candida albicans carried in the oral cavity of patients' dental prosthesis users in Brazil. Mycopathologia, 164, 255, 2007.

7. Samaranayake Y.H., Samaranayake L.P., Dassanayake R.S. et al.: Genotypic shuffling of sequential clones of Candida albicans in HIV-infected individuals with and without symptomatic oral candidiasis. J. Med. Microbiol., 52, 349, 2003.

8. Teanpaisan R. Niyombandith M., Pripatnanant P. et al.: Biotypes, genotypes and ketoconazole susceptibility of Candida albicans isolates from a group of Thai AIDS patients. New Microbiol., 31, 409, 2008.

9. Trtkova J., Pavlicek P., Ruskova L. et al.: Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation. BMC Microbiol., 9, 234, 2009.

10. Waltimo T.M.T., Dassanayake R.S., Orstavik D. et al.: Phenotypes and randomly amplified polymorphic DNA profiles of Candida albicans isolates from root canal infections in a Finnish population. Oral Microbiol. Immunol., 16, 106, 2001.

Downloads

Published

2025-04-09