Antioxidant activity of superoxide dismutase and glutathione peroxidase in cattle tissue
DOI:
https://doi.org/10.12923/Keywords:
superoxide dismutase, glutathione peroxidase, Taenia saginata, antioxidant, cattleAbstract
In this study the activity of two antioxidant enzymes, superoxide dismutase (EC 1.15.1.1) and glutathione peroxidase (EC 1.11.1.9) was examined in skeletal muscles from cattle infected with Taenia saginata. We observed a statistically significant increase (relative to the control value in uninfected cattle) in the level of enzymes in spectrophotometric analysis (superoxide dismutase, glutathione peroxidase) and western blot technique (superoxide dismutase). There were no significant differences in the activity of GPx in cattle tissue infected with cysticercus in comparison with the control healthy animals assessed with western blot technique.
Our results suggest that SOD and GPx enzymes are important in the host’s biochemical defence mechanisms during cysticercosis.
References
1. Balinska M.: Wolne rodniki w inwazji pasożytniczej. Wiad. Parazytol., 38, 22, 1992.
2. Boczon K., Hadas E., Wandurska-Nowak E., Derda M.: A stimulation of antioxidants in muscle of Trichinella spiralis infected rats. Acta Parasitol., 41, 138, 1996.
3. Chaible L.M., Alba-Loureiro T.C., Maia A.A.M. et al.: Effect of cysticercus celulosae on neutrophil function and death. Vet. Parasitol., 127, 129, 2005.
4. Deger Y., Ertekin A., Deger S., Mert H.: Lipid Peroxidation and Antioxidant Potential of Sheep Liver Infected Naturally with Distomatosis. Türkiye Parazitoloji Dergisi, 32, 23, 26, 2008.
5. Derda M., Hadas E.: Antioxidants and proteolytic enzymes in experimental trichinellosis. Acta Parasitol., 45, 361, 2000.
6. Derda M., Wandurska-Nowak E., Hadas E.: Changes in the level of antioxidants in the blood from mice infected with Trichinella spiralis. Parasitol. Res., 93, 210, 2004.
7. Dorny P., Praet N.: Taenia saginata in Europe. Vet Parasitol., 149, 22, 24, 2007.
8. Henkle K.J. et al.: Characterization and molecular cloning of a Cu/Zn superoxide dismutase from the human parasite Onchocerca volvulus. Infect Immun., 59, 2063, 2069, 1991.
9. Kolodziejczyk L., Siemieniuk E., Skrzydlewska E.: Fasciola hepatica: Effects on the antioxidative properties and lipid peroxidation of rat serum. Exp. Parasitol., 113, 48. 2006.
10. Kozłowska-Łój J., Rzymowska J.: Expression of superoxide dismutase (SOD) gene in blood cells and hepatocytes of intermediate hosts infected with Echinococcus granulosus (Batsch,1786). Annales UMCS sect. DDD, 19, 1, 53, 249, 2006.
11. Kozłowska-Łój J., Rzymowska J.: Prevalence of cysticercosis in cattle and pigs in the Lublin province in the years 1993-2004. Annales UMCS sect. DD, 61, 17, 143, 2006.
12. Lilić A. et al.: Activity of antioxidative defense enzymes in the blood of patients with liver echinococcosis. Vojnosanit Pregl., 64, 235, 240, 2007.
13. Ogunremi O., Benjamin J.: Development and field evaluation of a new serological test for Taenia saginata cysticercosis. Vet. Parasitol., 169, 93, 101, 2009.
14. Pugine S.M.P., Faria M.F, Maia A.A.M. et al.: Effect of cysticercus cellulosae fractions on the respiratory burst of pig neutrophils. Rev. Inst. Med. Tropl. Sao Paulo, 47, 94, 2005.
15. Scandrett B. et al.: Distribution of Taenia saginata cysticerci in tissues of experimentally infected cattle. Vet Parasitol., 164, 223, 231, 2009.
16. Štěrba J., Dyková I., Machnicka B.: Tissue reaction in the heart of cattle with a spontaneous and artificial cysticercus bovis infection. Folia Parasitol. (Praha), 26, 33, 1979.
17. Waloch M.: Cestode infections in Poland in 2006. Przegl. Epidemiol., 62, 355, 2008.
18. Waloch M.: Cestode infections in Poland in 2007. Przegl. Epidemiol., 63, 269, 2009.
Downloads
Published
Issue
Section
License
Copyright (c) 2010 Authors

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.