Kinetic characteristics of purified H+,K+-ATPase from parietal cells plasma membrane under stress-induced stomach ulcer

Authors

  • Eugenia Strotska Taras Shevchenko National University of Kyiv, Department of Biochemistry Author
  • Yana Raetska Taras Shevchenko National University of Kyiv, Department of Biochemistry Author
  • Sergey Mandryk Taras Shevchenko National University of Kyiv, Department of Biochemistry Author
  • Lyudmila Ostapchenko Taras Shevchenko National University of Kyiv, Department of Biochemistry Author

DOI:

https://doi.org/10.12923/

Keywords:

H+, K+-ATPase, parietal cells, stomach ulcer

Abstract

In the present study of kinetic parameters of purified H+,K+-ATPase of rats plasma membrane under stress-induced stomach ulcer were investigated. It was established that elution profile of the protein had one peak under control conditions and two peaks – under the stomach ulcer. Results of SDS-PAGE of purified enzyme visualized a single band, with an apparent molecular weight of about 97 kDa. Analysis of kinetic parameters of purified H+,K+-ATPase had the following features: decrease of Km and increase Vmax in ATP hydrolysis reaction, increase of K+-affinity and shift of enzyme pHopt to 5.0.

References

1. Bradford M.M.: A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem., 72, 248, 1976.

2. Golubchikov M.V.: Statistichniy oglyad zahvoryavannya naselennya Ukraini na xvorobu pechinki ta zhovchovuvidnuh shlyahiv. Suchasna Gastroenterologiya i Gematologiya, 2, 53, 2002.

3. Groisman S.D., Karevina T.G.: O vliyanii atropina na stressovie porazheniya slizistoi obolochki zheludka y krus. Bibl. ukaz. VINITI. Dep. Rucopisi, 12, 131, 1979.

4. Hersey S.J., Sachs G.: Gastric Acid Secretion, Physiological Reviews, 75, 1, 155, 1995.

5. Lacapere J., Robert J., Thomas-Soumarmon A.: Efficient solubilization and purification of the gastric H+,K+-ATPase for functional and structural studies. Biochem. J., 345, 239, 2000.

6. Laemmli U.: Cleavage of structural proteins during the assembly of the bacteriophage T4. Nature, 227, 680, 1970.

7. Sachs G.: Gastric H,K-ATPase as therapeutic target. Ann. Rev. Pharmacol. Toxicol., 28, 269, 1988.

8. Sachs G., Chang H.H., Rabon E.R. et al.: A nonelectrogenic H+ pump in plasma membranes of hog stomach. J. Biol. Chem., 251, 7690, 1976.

9. Tairov M.M., Bersimbaev P.I., Argutinskaya S.V. et al.: Kletochnaya lokalizaciya adenilatcyclaz, stimyliryemih gistaminom v slizistoi obolochke zheludka krus i ih rol v regulacii zheludochnoi secrecii. Biochimiya, 48, 1035, 1983.

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Published

2025-04-09

Issue

Vol. 23 No. 2 (2010): Annales UMCS, Pharmacia, Sectio DDD, Volume XXIII

Section

Artykuły

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