Structural biochemical assessment of the status of the nuclear apparatus of the rat spleen lymphoid cells under radiation treatment

Authors

  • Lyudmila Dragan Taras Shevchenko National University of Kyiv Author
  • Tetyana Andriychuk Taras Shevchenko National University of Kyiv Author
  • Lyudmila Ostapchenko Taras Shevchenko National University of Kyiv Author

DOI:

https://doi.org/10.12923/

Keywords:

apoptosis, parameter α, fluorescence microscopy, acridine orange, spleen lymphocytes, ionizing radiation

Abstract

Using two-color photometry of local regions of luminescent images of rat spleen lymphocytes and a new computer software for data processing, pyknotic events accompanied by condensation of nuclear material and its fragmentation leading to the formation of apoptotic bodies have been registered. Under IR exposure, a change in the integral index of the degree of DNA spiralization (parameters a [a=640/І530]) has been found to occur, which indicates structural damage in chromatin.

References

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Published

2025-04-09

Issue

Vol. 23 No. 2 (2010): Annales UMCS, Pharmacia, Sectio DDD, Volume XXIII

Section

Artykuły

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