Paclitaxel and apoptosis in breast cancer cells
DOI:
https://doi.org/10.12923/Keywords:
paclitaxel, apoptosis, breast cancer, caspases, microarray methodAbstract
Our analysis was connected with estimation of changes in expression of genes participating in apoptotic process under influence of paclitaxel applied in breast cancer cells in vitro.
Increase of expression of antiapototic genes after administration of paclitaxel in a dose 60 ng/ml requires explanation. These observation suggest that expression of antiapoptotic genes should not be so intense. It is possible that expression of antiapoptotic genes is less that proapoptotic. Another explanation suggests the levels of both group are similar, but because of unknown cell mechanism the prevalence achieve proapototic genes. It is possible that in this process has significance caspase activation, what leads to irreversible reaction cause degradation of cell structures.
Paclitaxel in a dose 60 ng/ml is activated opposing groups of pro and antiapoptotic genes. The probable reason of advantage apoptotic processes over antiapoptotic processes in breast cancer cells may be explained by activation significant numbers of ways connected with phosphorylation of Bcl-2 gene products.
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