Effect of osthole on the protective activity of carbamazepine and phenobarbital against maximal electroshock-induced seizures in mice
DOI:
https://doi.org/10.12923/Keywords:
Osthole, carbamazepine, phenobarbital, maximal electroshock seizure testAbstract
The aim of this study was to determine the effect of osthole on the anticonvulsant activity of two classical antiepileptic drugs (carbamazepine and phenobarbital) in the mouse maximal electroshock seizure model. Electroconvulsions were evoked in adult Albino Swiss mice by a current (50Hz, 500V, 0.2s stimulus duration) delivered via auricular electrodes. Acute adverse-effect profiles of the combination of osthole with carbamazepine and phenobarbital were measured in the chimney test (motor performance), passive avoidance task (long-term memory) and grip-strength test (skeletal muscular strength) in mice. Results indicate that osthole administered intraperitoneally (i.p.) at a dose of 200 mg/kg significantly elevated (by 41%; p<0.01) the threshold for electroconvulsions in mice. Osthole at lower doses of 50, 100 and 150 mg/kg had no significant impact on the threshold for electroconvulsions in mice. Osthole (50, 100 and 150 mg/kg, i.p.) did not significantly affect the protective action of carbamazepine and phenobarbital in the maximal electroshock-induced seizures in mice. Moreover, osthole in combination with carbamazepine and phenobarbital did not alter motor performance, long-term memory or skeletal muscular strength in experimental animals. The present study demonstrates that osthole, although elevated the threshold for electroconvulsions, had no significant effect on the anticonvulsant action of carbamazepine and phenobarbital in the mouse maximal electroshock-induced seizure model.
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