The influence of the short peptide of arginyl-alfa-aspartyl-lysyl-valyl-tyrosyl-arginine on the activity of NO-synthase system and processes of lipoperoxidation in experimental gastric lesions in rats
Keywords:
arginyl-alfa-aspartyl-lysyl-valyl-tyrosyl-arginine, gastroprotection, lipoperoxidation, NO-synthase systemAbstract
In the experiments on rats, under conditions of modeled ulcer of the stomach, the role of arginyl-alfa-aspartyl-lysyl-valyl-tyrosyl-arginine – Imunofan was investigated on the processes of lipoperoxidation and the status of the system of L-arginine/NOS/NO. It was shown that Imunofan action at the background of ulcerative lesions of the stomach caused a steep decline of the activity of NO-synthases, decrease of NO content in GM and increase of L-arginine concentration in the plasma of blood. The action of Imunofan at the background of blockage of iNOS with amine guanidine induced the inhibition of iNOS activity and a decrease of NO content in GM, whereas the concentration of L-arginine in the plasma of blood increased. Under conditions of combined action of Imunofan with L-arginine, the activity of NO-synthases reduced, the content of nitrogen oxide slightly increased, and the concentration of L-arginine in the plasma of blood was determined higher than in the control animals. The action of Imunofan also caused a decrease of the processes of lipoperoxidation and SOD activity.
References
1. Aleynikowa T.L. et al.: Medicyna, 128, 2000.
2. Belostotskiy N.I.: Yazveobrazovanie v slizistoy obolochke zheludka krys pod vliyaniyem katekholaminov. Patol. Fiziol. Eksp. Med., 1, 24, 1988.
3. Chevari S. et al.: Opredelenie antioksidantnyh parametrov krovi i ih diagnosticheskoe znachenie v pozhilom vozraste. Lab. Delo, 10, 9, 1991.
4. Green L.C., David A.W.: Analysis of nitrate, nitrite and (1515) nitrate in biological fluids. Anal. Biochem, 126, 131, 1982.
5. Ilic S. et al.: Stable gastric pentadecapeptide BPC 157 and insulin induced gastric lesions in rats. J. Phys. Pharm., 2, 40, 2009.
6. Ko J.K.S., Cho C.H.: CO-regulation of mucosal nitric oxide and prostaglandin in gastric adaptive cytoprotection. Inflamm. Res., 48, 471, 1999.
7. Korolyuk M.A. et al.: Metod opredeleniya aktivnosti katalazy. Lab. Delo, 1, 16, 1988.
8. Sklyarov O.Ya.: Periferiyni mekhanizmy regulacii procesiv cytoprotekcii u slyzoviy obolonci shlunka. ВМС, 2007.
9. Lebedeva V.V.: Problemy patogeneza i terapii imunnyh rasstrojstv. Moskva, 2002.
10. Samonina G.E. et al.: Tripeptid Pro-Gly-Pro i gomeostaz slizistoj obolochki zeludka. Neurochimia, 1, 128, 2008.
11. Sumbaev V.V., Yasinskaya I.M.: Vliyanie DDT na aktivnost sintazy oksida azota v pecheni, legkich i golovnom mozge. Sovrem. Probl. Toksikol, 3, 3, 2000.
12. Timirbulatov R.A., Seleznev E.I.: Metod povysheniya intensivnosti svobodnoradikalnogo okisleniya lipidsoderzashchih komponentov krovi i ego diagnostichskoe znachenie. Lab. Delo, 4, 209, 1981.
13. Trufanowa A.V. et al.: Gistomorfologicheskie charakteristiki uskorennogo zazhivlenia acetat- inducirovannih yazv pod vliyaniem gliprolinov. Bull. Eksp. Biol. Med., 2, 258, 2007.
14. Yadav S.K. et al.: The gastric ulcer-healing action of allylpyrocatechol is mediated by modulation of arginase metabolism and shift of cytokine balance. Eur. J. Pharmacol., 1-3, 106, 2009.
Downloads
Published
Issue
Section
License
Copyright (c) 2010 Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.
