Thin layer chromatography (TLC) and video-scanning in a new method for the analysis of angiotensin AT1 receptor antagonists, telmisartan and valsartan, in pharmaceuticals

Tadeusz Inglot; Anna Gumieniczek; Paulina Mączka

Authors

Tadeusz Inglot Department of Medicinal Chemistry, Medical University of Lublin, Poland Author https://orcid.org/0000-0003-1532-4430
Anna Gumieniczek Department of Medicinal Chemistry, Medical University of Lublin, Poland Author https://orcid.org/0000-0002-2503-5272
Paulina Mączka Department of Medicinal Chemistry, Medical University of Lublin, Poland Author

Keywords:

thin layer chromatography, video-scanning, angiotensin AT1 receptor antagonists, determination, pharmaceuticals

Abstract

Video-scanning procedure was performed for a new TLC method of quantitative analysis of two angiotensin AT₁receptor antagonists, telmisartan and valsartan, in pharmaceuticals. Determination was carried out using silica gel adsorbent, a mobile phase consisting of 1,4-dioxane-hexane-formic acid (99%) (5:5:0.1, v/v/v) and detection at 254 nm for the both drugs. Compact spots were obtained for telmisartan with R_F 0.27 ± 0.01 (mean ± SD) and for valsartan with R_F 0.39 ± 0.01. Calibration plots were linear with good correlation coefficients equal 0.9968 and 0.9966 for telmisartan and valsartan. The method was verified for robustness, precision and accuracy.

References

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Thin layer chromatography (TLC) and video-scanning in a new method for the analysis of angiotensin AT₁receptor antagonists, telmisartan and valsartan, in pharmaceuticals

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