Phenolic acids in the flowering herbs of Cirsium oleraceum (L.) Scop. growing in Poland

Authors

  • Małgorzata Kozyra Department of Pharmacognosy with Medicinal Plant Laboratory Author https://orcid.org/0000-0002-9645-8133
  • Renata Łoś Department of Pharmaceutical Microbiology, Medical University of Lublin, Poland Author
  • Anna Malm Department of Pharmaceutical Microbiology, Medical University of Lublin, Poland Author https://orcid.org/0000-0003-1503-7634
  • Kazimierz Głowniak Department of Pharmacognosy with Medicinal Plant Laboratory Author

Keywords:

Cirsium oleraceum (L). Scop., phenolic acids, antibacterial activity

Abstract

In this work phenolic acids in the methanol extract from the flowering herbs of Cirsium oleraceum (L.) Scop. were isolated and identified for the first time. The samples containing free phenolic acids and those released after acid and alkaline hydrolyses were investigated by 2D TLC on cellulose. After purification by SPE, samples were also analyzed by RP-HPLC. Three free phenolic acids were identified – p-coumaric, caffeic and protokatechuic acids. Moreover, after acid hydrolysis rosmarinic and syringic acids were detected, while after alkaline hydrolysis – ferulic, ellagic and syringic acids.  Using agar well diffusion method, no activity of the extract (20 mg/ml) against the panel of ten reference strains of Gram-positive and Gram-negative bacteria was found.

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Published

2010-12-30

Issue

Vol. 23 No. 4 (2010): Annales UMCS, Pharmacia, Sectio DDD, Volume XXIII

Section

Artykuły

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