Serine-threonine protein kinase activities under colitis-associated carcinogenesis development

Authors

  • Lyudmila Ostapchenko Taras Shevchenko Kyiv National University, Biological Department, Ukraine Author
  • Olesya Drobinska Taras Shevchenko Kyiv National University, Biological Department, Ukraine Author
  • Olga Kravchenko Taras Shevchenko Kyiv National University, Biological Department, Ukraine Author

Keywords:

ulcerative colitis, epitheliocytes, serine-threonine protein kinases, colitis-associated carcinogenesis

Abstract

Kinases are enzymes involved in mechanisms of ion transport, proliferation and differentiation control by phosphorylation of different protein substrates. The aim of the study was to research the serine-threonine protein kinase activities in membrane and cytoplasmic fractions of colon mucosa epithelial cell under colitis-associated carcinogenesis development.

References

1. Bradford M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Ann. Biochem., 72, 248, 1976.

2. Hunter T.: Protein phosphorylation. Part A. Protein kinase: assays, purification, antibodies, functional analysis, cloning and expression. Methods in enzymology. Academic Press. Inc., New York 1991.

3. Jian-Guo W., Dong-Fei W. et al.: A novel mouse model for colitis-associated colon carcinogenesis induced by 1,2-dimethylhydrazine and dextran sulfate sodium. World J. Gastroenterol., 10, 2958, 2004.

4. Ostapchenko L.I., Kravchenko O.O., Drobinskaya O.V.: Aktivnist Са2+- zalezhnych proteinkinaz v epiteliocitach slyzovoi obolonky shlunka shchuriv za umov rozvytku kolit-asociyovanogo kancerogenezu. Dopovidi NAN, 12, 154, 2008.

5. Parames S.: Increased protein kinase C activity in myotrophin-induced myocyte growth. Circ. Res., 82, 1173, 1998.

6. Podolsky D.K.: Inflammatory bowel disease. New Eng. J. Med., 347, 417, 2002.

7. Roediger W.E. et al.: Methods of preparing isolated colonic epithelial cells (colonocytes) for metabolic studies, Gut. 20, 484, 1979.

8. Rybalchenko V.K.: Structura i funkcii membran. Кyiv 1988.

9. Shabb J.B.: Physiological substrates of cAMP-dependent protein kinase. Chem. Rev., 101, 2381, 2001.

10. Zheng J. H. et. al.: Role of calcium signal in apoptosis and protective mechanism of colon cancer cell line SW480 in response to 5-aminolevulinic acid-photodynamic therapy. Ai Zheng, 6, 683, 2006.

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Published

2010-12-30