Natural inhibitors of metalloproteinases from various families of MA clan from wood degrading fungi

Abstract

In higher organisms changes in level of proteases and/or their inhibitors are connected not only with organism homeostasis, but also with diseases and illnesses. One of protease clan – MA, contain metalloproteases connected with pathological processes like arthritis, tumor diseases and multiple sclerosis (family of matrix metalloproteinase – MMPs from vertebrates) and bacterial infection processes (family of thermolysin and family of bacterial collagenase). Fungi are very interesting and promising new resources of proteolytic enzymes and their natural inhibitors. All catalytic classes of enzymes and some families of natural protease inhibitors were found in them. But the least known are fungal inhibitors of metalloproteinases  From some species of wood rotting fungi, obtained both from fruit bodies from nature and from laboratory, mycelial cultures, new inhibitors of few families from MA clan of metalloproteinases were extracted. Fractions active against metalloproteinases were extracted with few polar and nonpolar solvents. Inhibitory parameters against various families of metalloproteinases were characterized with chosen marker enzymes from concrete metalloproteinase family. Results obtained suggest that isolated substances are both organics (similar to hydrochinones and polyporenic acids from Piptoporus betulinus.), and peptides (low-molecular proteins or high-molecular oligopeptides). They have inhibitory activity both against MMPs and bacterial metalloproteinases. And they both are very promising bioactive substances (few extracts are really very active in comparison with available literature data).