The effect of protocatechuic acid on the tongue mucosa in rats exposed to n-nitrosomethylbenzylamine
DOI:
https://doi.org/10.12923/Keywords:
protocatechuic acid, N-nitrosomethylbenzylamine, experimental carcinogenesis, tongue, ratAbstract
The effect of natural plant-derived phenol – protocatechuic acid on lesions induced by carcinogen – N-nitrosomethylbenzylamine in the squamous epithelium of the tongue was investigated in rats. Carcinogen was applied for 3-8 weeks of the experiment (0.5 mg/kg/dose; 3 doses/week). Protocatechuic acid was administered with a daily diet in two doses 1000 or 2000 ppm for first 7 or 24 weeks of the experiment. Incidence and multiplicity of gross lesions on the dorsal surface of the tongue was the highest in rats exposed exclusively to carcinogen. Multiplicity of lesions was significantly lower in the group fed with diet containing protocatechuic acid in higher dose for the whole experiment. The most frequent lesion in the microscopic examination was hyperplasia of the squamous epithelium. Dysplasia and papillomas were rarely seen, while carcinomas were not observed. Chemoprevention activity of protocatechiuc acid in applied experimental model was confirmed.
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