Keywords
ELISA
TGF-β
IL-10
Abstract
The recent study assessed TGF-β and IL-10 as immunosuppressive cytokines of the cell mediated immunity (CMI) against Brucella in the serum of some brucellosis patients. Serum from 15 patients and 15 healthy individuals were obtained, and then cytokine concentrations above were investigated. Concentrations of TGF-β induced in the serum of acute patients (89.73 ± 15.55 ng/ml) were higher than healthy group (58.86 ± 17.89 ng/ml, P < 0.0001). While, no significant differences were found in the concentration of IL-10 between two groups. Based upon the findings of our study, the contribution of these cytokines to the pathobiology of infection had been shown. Therefore, assessment of immunosuppressive cytokines as TGF-β and IL-10 levels may be a potential diagnostic strategy for patients with brucellosis.References
1. Skendros P. et al.: Cell-mediated immunity in human brucellosis. Microbes Infect. 2011;13:134.
2. Gomez G. et al.: Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis. Front Cell Infect Microbiol.,3,1,2013.
3. Mangodt TC. et al.: The role of Th17 and Treg responses in the pathogenesis of RSV infection. Pediatr Res.,78,483,2015.
4. Mohammadnia-Afrouzi M. et al.: Altered microRNA Expression and Immunosuppressive Cytokine Production by Regulatory T Cells of Ulcerative Colitis Patients. Immunol Invest. 2016;45:63.
5. López-Abente J. et al.: Functional Mechanisms of Treg in the Context of HIV Infection and the Janus Face of Immune Suppression. Front Immunol. 2016;7:192.
6. Seeger P. et al.: The TGF-β superfamily in dendritic cell biology. Cytokine Growth Factor Rev. 2015;26:647.
7. Couper KN. et al.: IL-10: the master regulator of immunity to infection. J Immunol. 2008;180:5771.
8. Sabat R. et al.: Biology of interleukin-10. Cytokine Growth Factor Rev. 2010;21:331.
9. Beswick EJ. et al.: Role of Gastric Epithelial Cell-Derived Transforming Growth Factor β in Reduced CD4(+) T Cell Proliferation and Development of Regulatory T Cells during Helicobacter pylori Infection. Infect Immun. 2011;79:2737.
10. Omer FM. et al.: Activation of Transforming Growth Factor β by Malaria Parasite-derived Metalloproteinases and a Thrombospondin-like Molecule. J Exp Med. 2003;198:1817.
11. Gough NR: Enhancing and Inhibiting TGF-β Signaling in Infection. Science Signaling.8,ec9,2015.10.1126/scisignal.aaa6549
12. de Figueiredo P. et al.: Pathogenesis and Immunobiology of Brucellosis: Review of Brucella–Host Interactions. Am J Pathol. 2015;185:1505.
13. Bahador A. et al.: Frequencies of CD4+ T Regulatory Cells and their CD25 high and FoxP3 high Subsets Augment in Peripheral Blood of Patients with Acute and Chronic Brucellosis. Osong Public Health Res Perspect. 2014;5:161.10.1016/j.phrp.2014.04.008
14. Skendros P. et al.: Diminished percentage of CD4+ T-lymphocytes expressing interleukine-2 receptor alpha in chronic brucellosis. J Infect. 2007;54:192.
15. Elfaki MG., Al-Hokail AA: Transforming growth factor β production correlates with depressed lymphocytes function in humans with chronic brucellosis. Microbes Infect. 2009;11:1089.
16. Sadeghi M. et al.: Cytokine expression during early and late phase of acute Puumala hantavirus infection. BMC Immunol. 2011;12:1.
17. Guyot-Revol V. et al.: Regulatory T cells are expanded in blood and disease sites in patients with tuberculosis. Am J Respir Crit Care Med. 2006;173:803.
18. Rodríguez-Zapata M. et al.: Human brucellosis is characterized by an intense Th1 profile associated with a defective monocyte function. Infect Immun. 2010;78:3272.
19. Chaiyaroj SC. et al.: Reduced levels of transforming growth factor-β1, interleukin-12 and increased migration inhibitory factor are associated with severe malaria. Acta Trop. 2004;89:319.
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