Identification of polyamidoamine dendrimers (PAMAM-NH2) by ESI-Q-TOF method

Authors

  • Robert Skibiński Department of Medicinal Chemistry, Medical University of Lublin, Poland Author
  • Magdalena Markowicz Author
  • Paweł Szymański Department of Pharmaceutical Chemistry and Drug Analysis, Medical University, Łódź, Poland Author
  • Elżbieta Mikiciuk-Olasik Department of Pharmaceutical Chemistry and Drug Analysis, Medical University, Łódź, Poland Author

DOI:

https://doi.org/10.12923/j.2084-980X/25.3/a.15

Keywords:

PAMAM, dendrimers, ESI, TOF, mass spectrometry

Abstract

The method of identification of poly(amidoamine) dendrimers (PAMAM-NH2) G1.0, G2.0 and G3.0 generation with the use of high resolution mass spectrometry and hybrid quadrupole-time-of-flight analyzer with electrospray ionization (ESI-Q-TOF) was developed. The analysis was performed in positive ionization mode in the range m/z 100-3200 where MS spectra (TOF) as well as fragmentation spectra (MS/MS) were recorded simultaneously. The investigated substances were injected to the MS analyzer by the use of UHPLC system in a direct way (with no chromatographic separation). After deconvolution MS spectra were used for accuracy estimation of the mass of the analyzed dendrimers (PAMAM G1.0 - 1430.0153 Da, PAMAM G2.0 - 3256.2696 Da, PAMAM G3.0 - 6908.6850 Da). Registered fragmentation spectra were used for the confirmation of the structure of the analyzed three generation PAMAM-NH2 dendrimers.

Published

2012-07-01

Issue

Vol. 25 No. 3 (2012): Current Issues of Pharmacy and Medical Sciences

Section

Artykuły

License

Copyright (c) 2025 Robert Skibiński, Magdalena Markowicz, Paweł Szymański, Elżbieta Mikiciuk-Olasik (Autor)

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.

How to Cite

Skibiński, R., Markowicz, M., Szymański, P., & Mikiciuk-Olasik, E. (2012). Identification of polyamidoamine dendrimers (PAMAM-NH2) by ESI-Q-TOF method. Current Issues in Pharmacy and Medical Sciences, 25(3), 286-293. https://doi.org/10.12923/j.2084-980X/25.3/a.15