Biological activity of extracts from the leaves and roots of Jovibarba sobolifera (Sims.) Opiz.

Katarzyna Szewczyk; Anna Bogucka-Kocka; Tadeusz Krzaczek

Authors

Katarzyna Szewczyk Department of Pharmaceutical Botany, Medical University of Lublin, Poland Author https://orcid.org/0000-0003-4884-9110
Anna Bogucka-Kocka Department of Pharmaceutical Botany, Medical University of Lublin, Poland Author https://orcid.org/0000-0001-8473-7429
Tadeusz Krzaczek Medical Institute, State High School of Informatics and Enterprise, Lomza, Poland Author

Keywords:

cytotoxic activity, apoptosis, Allium test, Jovibarba sobolifera (Sims.) Opiz, Crassulaceae

Abstract

The fractions of polysaccharides from rosette leaves and roots, and ethanolic extracts from fresh and dried leaves of Jovibarba sobolifera (Sims.) Opiz were investigated. All isolated polysaccharide fractions were mainly composed of glucose, rhamnose and galactose. Biological activity testing using the Allium test showed mitostatic activity for all fractions. It was discovered that mucilage contained in Jovibarba sobolifera impedes the linear growth of onion roots and causes mitostatic activities disorder. It appeared that mucilage extracted from herbs is more active. Additionally, having stimulated the Jurkat line cells by examined fractions, the evaluation of cytostatic activity and the evaluation of the number of cells in the early stage of apoptosis, was carried out. Water solutions of mucilage of herbs and roots, and ethanol extracts made of dried and fresh herbs were subjected to examination. The conducted tests revealed that ethanol extracts of Jovibarba sobolifera had the strongest effect. Cytostatic activity of examined fractions (polysaccharides and ethanolic extracts) were stated using the test with trypan blue (0.4% aqueous solution of blue) also.

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Biological activity of extracts from the leaves and roots of Jovibarba sobolifera (Sims.) Opiz.

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