Interaction between drug Ukrain and Simvastatin in vitro studies

Abstrakt

The aim of this study was to investigate the interaction between the drugs Ukrain and Simvastatin in vitro studies. The research was conducted on green monkey kidney cells (GMK). The experiment used the drug Ukrain (at concentrations of 50 μmol/l and 150 μmol/l), the drug Simvastatin (at concentrations of 100 μmol/l and 300 μmol/l), and the combination of these two drugs in the ratio of 1:1, 1:3 and 3:1. The cytotoxicity of both drugs were evaluated using a Cytotoxicity Detection Kit (LDH), while cell viability was determined using the MTT assay (based on the MTT assay protocol INVITTOX n°17, ECVAM), after 6, 12 and 24 h incubation with the tested drugs. Cytotoxicity of drug Ukrain (50 μmol/l) and Simvastatin (100 μmol/l) alone was observed after 6 h incubation of GMK cells with both drugs., while, Ukrain in combination with Simvastatin (300 μmol/l) showed cytotoxicity only after 12 h of incubation. However, cytotoxicity of the drug Ukrain (150 μmol/l), together with Simvastatin (100 μmol/l) was observed after 6 and 12 h incubation with GMK cells. The greatest inhibition growth of green monkey kidney cells was noted after 24 h incubation with all concentrations of these drugs.