Qualitative and quantitative analysis of diosgenin in pure substance by TLC-densitometry
DOI:
https://doi.org/10.12923/j.2084-980X/25.3/a.20Słowa kluczowe:
diosgenin, TLC-densitometry, thin-layer chromatographyAbstrakt
A simple and sensitive TLC-densitometric method for qualitative and quantitative analysis of diosgenin in pure sample was elaborated. Satisfactory results of the densitometric analysis of examined compound was obtained using chloroform-acetone in volume composition 40:10 (v/v) as a mobile phase and by means of the chromatographic plates precoated with silica gel 60F254 and silica gel 60. Visualization of the diosgenin spots was achieved by dipping the plates into 10% ethanol solution of phosphomolybdic acid and 10% ethanol solution of sulfuric acid respectively. The limit of detection (LOD) and the limit of quantification (LOQ) of diosgenin investigated under different chromatographic conditions was determined. The results obtained in this work indicate that, the elaborated method is suitable for densitometric analysis of diosgenin but only in pure sample.
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Prawa autorskie (c) 2012 Autorzy
Praca jest udostępniana na licencji Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 Unported License.
