Determination of coumarins from aerial part of two Artemisia species
DOI:
https://doi.org/10.12923/j.2084-980X/26.1/a.14Słowa kluczowe:
Accelerated Solvent Extraction (ASE), Asteraceae, Artemisia gmelinii Webb ex Stechmann, Artemisia umbelliformis Lam., coumarins, HPLC-DAD analysis methodAbstrakt
The methanolic and methanolic-water extracts were received from a blooming aerial part (herb) of two species: Artemisia gmelinii Webb ex Stechmann and Artemisia umbelliformis Lam. Two methods were used for extracting the plant material: Accelerated Solvent Extraction (ASE) and Soxhlet Extraction. The analysis of samples was performed by the HPLC-DAD method. All obtained extracts were subjected to the process of the solid phase extraction. Samples for HPLC-DAD analysis were purified using C-18 (Octadecyl, 500 mg) Baker Bond (J.T. Baker, USA) SPE-micro-columns. The quantitative indications of individual constituents (coumarins) were converted into the content of scopoletin.
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