Zastosowanie nowoczesnych technik ekstrakcyjnych w izolacji roślinnych metabolitów wtórnych – praca przeglądowa.
Słowa kluczowe:
Ekstrakcja, ekstrakcja nadkrytyczna, ekstrakcja ciśnieniowa, ekstrakcja wspomagana mikrofalamiAbstrakt
Nieustające poszukiwania nowych leków skutecznych w walce z nieuleczalnymi chorobami stawia coraz wyższe wymagania technikom ekstrakcji, separacji i analizy materiału roślinnego. Ekstrakcja ciał czynnych z materiału biologicznego wymaga zaawansowanych technik ekstrakcji. Dobór właściwych technik ekstrakcji zapewnia pełny odzysk metabolitów wtórnych zawartych w różnych częściach substancji roślinnej w zadowalających ilościach, z zachowaniem krótkiego czasu ekstrakcji oraz z zastosowaniem nietoksycznych rozpuszczalników. W niniejszym opracowaniu wnikliwie scharakteryzowano trzy techniki ekstrakcji. Ekstrakcja nadkrytyczna, przyspieszona ekstrakcja ciśnieniowa oraz ekstrakcja wspomagana promieniowaniem mikrofalowym zdecydowanie przewyższają klasyczne techniki wytrawiania substancji roślinnych. Ponadto przedstawiono wybrane aspekty doboru warunków zapewniających jak najwyższy stopień odzysku metabolitów wtórnych z roślin należących do różnych rodzin.
Bibliografia
1. Benthin B., Danz H., Hamburger M.: Presurized liquid extraction of medicinal plants. J. Chrom. A, 837, 1-2, 211, 2009.
2. Borivoj K., Mikelova R., Adam V. et al.: Liquid chromatographic–mass spectrometric determination of genistin and daidzin in soybean food samples after accelerated solvent extraction with modified content of extraction cell. Anal. Chim. Acta, 517, 1, 2004.
3. Brachet A., Rudaz S., Mateus L. et al.: Optimisation of accelerated solvent extraction of cocaine and benzoylecgonine from coca leaves. J. Sep. Sc., 24, 865, 2001.
4. Brachet A., Christen P., Veuthey J.L.: Focused microwave-assisted extraction of cocaine and benzoylecgonine from coca leaves. Phytochem. Anal., 13, 162, 2002.
5. Cazes J. editor (2010). Encyclopaedia of Chromatography. Boca Raton, FL: CRC Press.
6. Chen J., Wang F., Liu J. et al.: Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections. Anal. Chim. Acta, 619, 180, 2008.
7. Chen J., Wang F., Liu J. et al.: Analysis of alkaloids in Coptis chinensis Franch by accelerated solvent extraction combined with ultra performance liquid chromatographic analysis with photodiode array and tandem mass spectrometry detections. Anal. Chim. Acta, 613, 184, 2008.
8. Cho S.K., Abd El-Aty A.M., Choi J.H. et al.: Optimized conditions for the extraction of secondary volatile metabolites in Angelica roots by accelerated solvent extraction. J. Pharm. Biomed. Anal., 44, 1154, 2007.
9. Ericsson M., Colmsjo A.: Dynamic microwave-assisted extraction. J. Chrom. A, 877, 141, 2000.
10. Fadel H., Marx F., El-Sawy A., El-Gorab A.: Effect of extraction techniques on the chemical composition and antioxidant activity of Eucalyptus camaldulensis var. Brevirostris leaf oils. Zeitschrift fur Lebensmittel-Unterschung und -Forschung A, 208, 212, 1999.
11. Font N., Hernandez F., Hogendoorn E.A. et al.: Microwave-assisted solvent extraction and reversed-phase liquid chromatography–UV detection for screening soils for sulfonylurea herbicides. J. Chrom. A, 798, 179, 1998.
12. Goli A.H., Barzegar M., Sahari M.A.: Antioxidant activity and total phenolic compounds of pistachio (Pistachia vera) hull extracts. Food Chem., 92, 521, 2005.
13. Hamburger M., Baumann D., Adler S.: Supercritical carbon dioxide extraction of selected medicinal plants – effects of high pressure and added ethanol on yield of extracted substances. Phytochem. Anal., 15, 46, 2004.
14. Heaton D.M., Bartle K.D., Rayner C.M., Clifford A.A.: Applications of supercritical fluid extraction in food analysis. J. High Resol. Chromatogr., 16, 666, 1993.
15. Hossain M.B., Barry-Ryan C., Martin-Diana A.B., Brunton N.P.: Optimisation of accelerated solvent extraction of antioxidant compounds from rosemary (Rosmarinus officinalis L.), marjoram (Origanum majorana L.) and oregano (Origanum vulgare L.) using response surface methodology. Food Chem., 126, 1, 339, 2010.
16. Huang B., Lei Y., Tang Y. et al.: Comparison of HS-SPME with hydrodistillation and SFE for the analysis of the volatile compounds of Zisu and Baisu, two varietal species of Perilla frutescens of Chinese origin. Food Chem., 125, 268, 2011.
17. Hua-Ying Z., Chun-Zhao L.: Microwave-assisted extraction of solanesol from tobacco leaves. J. Chrom. A, 1129, 135, 2006.
18. Kaufmann B., Christen P., Veuthey J.L.: Parameters affecting microwave-assisted extraction of withanolides. Phytochem. Anal., 12, 327, 2001.
19. Kocjan R. editor (2002): Chemia Analityczna. Tom 2. Analiza instrumentalna. Warszawa: PZWL.
20. Kratchanova M., Pavlova E., Panchev I.: The effect of microwave heating of fresh orange peels on the fruit tissue and quality of extracted pectin. Carbohyd. Polym., 56, 181, 2004.
21. Lafka T.I., Lazou A.E., Sinanoglou V.J., Lazos E.S.: Phenolic and antioxidant potential of olive oil mill wastes. Food. Chem., 125, 92, 2011.
22. Lasekan O., Abbas K.: Analysis of volatile flavour compounds and acrylamide in roasted Malaysian tropical almond (Terminalia catappa) nuts using supercritical fluid extraction. Food Chem. Toxicol, 48, 2212, 2010.
23. Li Y., Pang T., Guo Z. et al.: Accelerated solvent extraction for GC-based tobacco fingerprinting and its comparison with simultaneous distillation and extraction. Talanta, 81, 650, 2010.
24. Lin M.C., Tsai M.J., Wen K.C.: Supercritical fluid extraction of flavonoids from Scutellariae Radix. J. Chrom., A 830, 387, 1999.
25. Ling J.Y., Zhang G.Y., Cui Z.J., Zhang C.K.: Supercritical fluid extraction of quinolizidine alkaloids from Sophora flavescens Ait. and purification by high-speed counter-current chromatography. J. Chroma. A, 1145, 123, 2007.
26. Liu B., Shen B., Guo F., Chang Y.: Optimization of supercritical fluid extraction of dl-tetrahydropalmatine from rhizome of Corydalis yanhusuo W.T. Wang with orthogonal array design. Sep. Purif. Techn., 64, 242, 2008.
27. Liu J., Lin S., Wang Z. et al.: Supercritical fluid extraction of flavonoids from Maydis stigma and its nitrite-scavenging ability. Food Bioprod. Process. 2010. ARTICLE IN PRESS FBP-176.
28. Markom M., Hasan. M., Wan Daud W.R. et al.: Extraction of hydrolysable tannins from Phyllanthus niruri Linn. Effects of solvents and extraction methods. Sep. Purif. Tech., 52, 487, 2007.
29. Min G., Bao-Zhen S., Chun-Zhao L.: Dynamic microwave-assisted extraction of flavonoids from Saussurea medusa Maxim. cultured cells. Biochem. Eng. J., 32, 79, 2006.
30. Molins C., Hogendoorn E.A., Heusinkveld H.A.G. et al.: Microwave assisted solvent extraction (MASE) of organochlorine pesticides from soil samples. Int. J. Environ. Stud., 68, 155, 1997.
31. Mroczek T., Mazurek J.: Pressurized liquid extraction and anticholinesterase activity-based thin-layer chromatography with bioautography of Amaryllidaceae alkaloids. Anal Chim. Acta, 633, 188, 2009.
32. Mukhopadhyay M. editor (2000): Natural Extracts Using Supercritical Carbon Dioxide. Bombay, India: CRC Press.
33. Murga R., Ruiz R., Beltran S., Cabezas J.L.: Extraction of natural complex phenols and tannins from grape seeds by using supercritical mixtures of carbon dioxide and alcohol. J. Agricult. Food Chem., 48, 3408, 2000.
34. Palma M., Taylor L.T., Varela R.M. et al.: Fractional extraction of compounds from grape seeds by supercritical fluid extraction and analysis for antimicrobial and agrochemical activities. J. Agric. Food Chem., 47, 5044, 1999.
35. Pereira C.G., Marques M.O.M., Barreto A.S. et al.: Extraction of indole alkaloids from Tabernaemontana catharinensis using supercritical CO2+ethanol: an evaluation of the process variables and the raw material origin. J. Supercrit. Fluids, 30, 51, 2004.
36. Qian Z.M., Lu J., Gao Q.P., Li S.P.: Rapid method for simultaneous determination of flavonoid, saponins and polyacetylenes in Folium Ginseng and Radix Ginseng by pressurized liquid extraction and high-performance liquid chromatography coupled with diode array detection and mass spectrometry. J. Chrom. A, 1216, 3825, 2009.
37. Richter B.E., Jones B.A., Ezzell J.L., Porter N.L.: Accelerated solvent extraction: A technique for sample preparation. Analyt. Chem., 68, 1033, 1996.
38. Rodrigues R.F., Tashima A.K., Pereira R.M.S. et al.: Coumarin solubility and extraction from emburana (Torresea cearensis) seeds with supercritical carbon dioxide. J Supercrit. Fluids, 43, 375, 2008.
39. Romanik G., Gilgenast E., Przyjazny A., Kaminski M.: Techniques of preparing plant material for chromatographic separation and analysis. Journal of Biochemical and Biophysical Analysis, 70, 253, 2007.
40. Rostagno M.A., Palma M., Barroso C.G.: Microwave assisted extraction of soy isoflavones. Anal. Chim. Acta, 588, 274, 2007.
41. Shi Ong E.: Extraction methods andchemical standardization of botanicals and herbal preparations. J. Chrom. B, 812, 23, 2004.
42. Skalicka-Wozniak K., Widelski J., Glowniak K.: Plant materials in modern pharmacy and methods of their investigations. In: Waksmundzka-Hajnos M., Sherma J., Kowalska T. editors (2008): Thin Layer Chromatography in Phytochemistry. Boka Raton/Florida/USA: CRC Press Taylor & Francis Group, 641-679.
43. Sparr Eskilsson C., Bjorklund E., Mathiasson M. et al.: Microwave-assisted extraction of felodipine tablets. J. Chrom. A, 840, 59, 1999.
44. Sparr Eskilsson C., Bjorklund E.: Analytical-scale microwave-assisted extraction. J. Chrom. A, 902, 227, 2000.
45. Vichi S., Santini C., Natali N. et al.: Volatile and semi-volatile components of oak wood chips analysed by Accelerated Solvent Extraction (ASE) coupled to gas chromatography–mass spectrometry (GC–MS). Food Chem., 102, 1260, 2007.
46. Tena M.T., Valcarcel M., Hidalgo P.J., Ubera J.L.: Supercritical Fluid Extraction of natural antioxidants from rosemary: comparison with Liquid Solvent Sonication. Anal. Chem., 69, 521, 1997.
47. Tsuda T., Mizuno K., Ohshima K. et al.: Supercritical carbon dioxide extraction of antioxidative component from tamarind (Tamarindus indica L.) seed coat. J. Agric. Food Chem., 43, 2803, 1995.
48. Vandenburg H.J., Clifford A.A., Bartle K.D. et al.: Comparison of pressurized fluid extraction and microwave assisted extraction with atmospheric pressure methods for extraction of additives from polypropylene. Analyst, 124, 397, 1999.
49. Waksmundzka-Hajnos M., Petruczynik A., Dragan A. et al.: Influence of the extraction mode on the yield of some furanocoumarins from Pastinaca sativa fruits. J. Chrom. B, 800, 181, 2004
50. Wu S.J., Tsai J.Y., Chang S.P. et al.: Supercritical carbon dioxide extra exhibits enhanced antioxidant and anti-inflammatory acticities of Physalis peruviana. J. Ethnopharm, 108, 407, 2006.
51. Zancan K.C., Marques M.O.M., Petenate A.J., Meireles M.A.A.: Extraction of ginger (Zingiber officinale roscoe) oleoresin with CO2 and co-solvents: a study of the antioxidant activity of the extracts. Journal of Supercritical Fluids, 24, 57, 1999.
52. Zhang L., Liu Z.: Optimization and comparison of ultrasound/microwave assisted extraction (UMAE) and ultrasonic assisted extraction (UAE) of lycopene from tomatoes. Ultrason. Sonochem, 15, 731, 2008.
53. Zhang Y., Li S.F., Wu X.W.: Pressurized liquid extraction of flavonoids from Houttuynia cordata Thunb. Sep. Purif. Techn.. 58, 305, 2008.
54. Zill-e-Huma, Viane A.M., Maingonnat J.F., Chemat F.: Clean recovery of antioxidant flavonoids from onions: Optimising solvent free microwave extraction method. J. Chrom. A, 1216, 7700, 2009.
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