Abstract
In the present publication experiments were concentrated on elaborating a simple method of separation standards of monosaccharides and choosing the best practically applied method of derivation. Sugars and their alcohol derivatives were analyzed. Six methods of monosaccharides detection were tested. Two stationary phases with several eluents were investigated. The method was used to analyze hydrolyzed monosaccharides from Malvae arborae flos polysaccharides. The samples were hydrolyzed into monosaccharide units with an acidic reagent – sulfuric acid (VI). On silica stationary phase, galactose was identified in hydrolysate of one fraction of Malve arborae polysaccharides. Investigations of monosaccharides separation with the use of HPTLC-Diol stationary phases was conducted for the first time. The best separation was achieved with a mixture of 1-propanol and ethyl acetate as a mobile phase. In this chromatographic set glucose was identified.
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