Abstract
In the present work, application of NP-TLC method for determination of the identity of dehydrocholic acid in selected pharmaceuticals containing dehydrocholic acid such as: Raphacholic C and Raphacholin Forte was estimated. Dehydrocholic acid was extracted from pharmaceutical formulations using methanol. Of all applied chromatographic conditions the best which allowed for obtaining optimal RF values of dehydrocholic acid are: the mixture of n-hexane–ethyl acetate–acetic acid in the following volume compositions: 25:20:5, 23:25:2, 22:22:6 as the mobile phases used and the chromatographic plates (E. Merck, Art. 1.05715, Art. 1.05554, Art.1.05553) and also chloroformacetone –acetic acid in volume composition 40:8:2 and chromatographic plates (E. Merck, Art. 1.05715 and Art. 1.05554). On the basis of the obtained results it was stated that the elaborated NPTLC method can be used in routine control of the quality of dehydrocholic acid pharmaceuticals.
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