Development and validation of a capillary electrophoresis method for the determination of lamotrigine in pharmaceutical dosage form

Abstract

A simple, rapid capillary electrophoretic (CE) method has been established and validated for determination of  antiepileptic drug lamotrigine, pure substance and in tablets. The method was developed by employing fused silica capillary with an effective length of 80 cm and internal diameter of  75 µm. 0.067 M phosphate buffer with pH 4.80 was used for lamotrigine analysis. The recommended applied voltage, capillary temperature and injection time were 30 kV, 30°C and 10 s, respectively. Detection was followed by direct absorptiometric measurements at 210 nm. Developed method was linear in the range of 5–100 µg/mL and had good correlation coefficient 0.9996. Intra- and inter-day precision, calculated as relative standard deviation (RSD), were better than 2.5 %. The proposed CE method was successfully applied to the assay of lamotrigine in pharmaceutical formulation. Excipients present in the tablets did not interfere in the assay.