Chemical composition and antioxidant activity of the essential oil of hyssop (Hyssopus officinalis L. ssp. officinalis). Part II. Free radical scavenging properties

Tomasz Baj; Elwira Sieniawska; Radosław Kowalski; Łukasz Świątek; Małgorzata Modzelelewska; Tadeusz Wolski

Authors

Tomasz Baj Dept. of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, Poland Author https://orcid.org/0000-0001-7543-6771
Elwira Sieniawska Dept. of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, Poland Author https://orcid.org/0000-0003-4773-0329
Radosław Kowalski Central Laboratory of Agroecology, University of Life Sciences in Lublin, Poland, Dept. of Analysis and Evaluation of Food Quality, University of Life Sciences in Lublin, Poland Author
Łukasz Świątek Deptment of Virology, Medical University of Lublin, Poland Author https://orcid.org/0000-0003-1626-680X
Małgorzata Modzelelewska Deptment of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, Poland Author
Tadeusz Wolski Deptment of Pharmacognosy with Medicinal Plant Laboratory, Medical University of Lublin, Poland Author

Keywords:

Hyssopus officinalis L., hyssop, essential oil, DPPH, TLC-DPPH, antioxidant activity

Abstract

In the presented work parallel derivatisation with DPPH and Vanlin reagent on the same plate was shown. The investigated sample was essential oil obtained from Hyssopus officinalis L. aerial parts. Parallel derivatisation enabled identification of active, containing antioxidants fraction, which R_f was 0.52. The antioxidant activity of essential oil was examined against the stable free radical DPPH (2,2–diphenyl-1-picrylhydrazyl-hydrate). The antioxidant activity was expressed by using parameter EC₅₀ and also compared with TEAC (Trolox). The results showed that the oil isolated from Hyssopus officinalis L. has low antioxidant activity EC₅₀value 21.13 mg/ mL and Trolox Equivalent value 0.029 mM.

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