Development and comparison of HPLC method with fluorescence and spectrophotometric detections for determination of sparfloxacin
Keywords:
HPLC, UV-fluorescent detection, sparfloxacinAbstract
An attempt was made to develop a simple, rapid, specific, precise and accurate RP-HPLC method with combined fluorescence and spectrophotometric detection and with simple mobile phase for determination of sparfloxacin (SPA). The comparison of both detection methods showed no significant differences with reference to precision and accuracy. A method thus developed was applied for the quantification of sparfloxacin in pure substance. The method was validated according to the ICH guidelines in respect to linearity, limit of detection, precision, accuracy, robustness, and selectivity. The developed HPLC method was successfully applied for assay of SPA pharmaceutical formulation to check the applicability of the proposed methodology.
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