Quantitative analysis of felbamate in pharmaceutical preparationby thin-layer chromatography with densitometric UV detection

Abstract

A new, simple, rapid, precise and accurate thin-layer chromatography method with densitometric detection was developed for the determination of felbamate in pharmaceutical preparations. The analysis was performed on silica gel 60 F₂₅₄ plates in horizontal chambers with acetone-chloroformacetic acid (59:40:1, v/v) as mobile phase. Densitometric assay was performed at 205 nm. The active substance was extracted from tablets with methanol. Calibration plot was constructed in the range 6.0–60.0 μg/spot with good correlation coefficient (r=0.9990). Intra- and inter-day precision, calculated as relative standard deviation (RSD), was better than 1.5%. The mean ± SD recovery from commercially available tablets was 100.05 ± 0.36% (n=6). Tablet excipients did not interfere with the chromatography.