Methods of apoptosis detection
Keywords:
apoptosis, methods of detectionAbstract
The aim of present study was analyze of methods of apoptosis detection. We described methods detecting single apoptotic cells and their populations. In those methods we showed: single cell gel electrophoresis (SCGE), apoptotic cell identification using transmission electron microscopy (TEM) and light microscopy; method based on assessment of changes in the apoptotic cell membrane; method based on Caspase 3 activity examination. The other methods exanimate releasing of cytochrome C from the mitochondria and detecting of DNA fragmentation. We described as well flow cytometry (flow cytophotometry). The manuscript was the most focused on immunohistochemical method.
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