Keywords
melanocytes
melanogenesis
tyrosinase
Abstract
The aim of this work was to examine the impact of ketoprofen on melanogenesis in cultured human normal melanocytes (HEMn-DP). The WST-1 assay was used to detect ketoprofen cytotoxic effect. It has been demonstrated that ketoprofen induces the loss of melanocytes viability in a concentration–dependent manner. The value of EC50 was found to be 1 mM. It has also been shown that ketoprofen causes inhibition of tyrosinase activity and reduces melanin content in human epidermal melanocytes. The demonstrated inhibitory effect of ketoprofen on melanization process in melanocytes in vitro may explain the potential role of melanin biopolymer in the mechanisms of undesirable phototoxic effects of this drug in vivo, as a result of its accumulation in pigmented tissues.
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