Abstract
We have demonstrated that ketoprofen and paracetamol form stable complexes with melanin and the amount of drug bound to the polymer increases with the increase of the initial drug concentration and the incubation time. The analysis of drugs binding to melanin has shown that at least two classes of independent binding sites must be implicated in these complexes formation: strong binding sites (n1) with the association constant K1~ 4-6•105 M-1 and weak binding sites (n2) with K2~2•104 M-1. The ability of the analyzed drugs to form complexes with melanin in vitro may be one of the reasons for their phototoxic side-effects in vivo, as a result of their accumulation in melanin in the skin.
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