The pregnancy as a inducting factor of intrinsic pathway of apoptosis in kidney’s cells of female rats

Authors

  • Agnieszka Pedrycz Department of Histology and Embryology Medical University in Lublin Author
  • Piotr Siermontowski Department of Maritime & Hyperbaric, Medicine Military Institute of Medicine, Gdynia Author
  • Marcin Orłowski Centrum of Diagnosis and Therapy of Digestive System’s Diseases Gdynia Author

Keywords:

caspase 9, programmed cell death, kidney, pregnancy

Abstract

The aim of this study was immunohistochemical evaluation of intrinsic pathway of apoptosis in renal epithelial cells in pregnant rats including time.

We evaluated in immunohistochemical way expression of the initiating caspase 9 – activated during apoptosis in protein complex-apoptosome.

Rats used in this experiment was divided into 4 groups – 8 individuals each: I group – female rats which was fertilized and decapitated in 10 day of pregnancy, II group female rats which was fertilized and decapitated in 20 day of pregnancy III group female rats which was fertilized and decapitated in 10 day after delivery VI group – control – female rats which decapitated in 30 day of experiment.

Specimens of kidneys taken to examine we analyzed in immunohistochemical standard three-step method detecting caspse 9 protein.

Immunohistochemical positive reaction increased in all experimental groups compared to control.

Pregnancy – induced hormonal changes in rats body activated apoptosis of renal cells via intrinsic pathway with active caspase 9. This pathway was the most intensive 10th day of pregnancy.

References

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Published

2011-11-24

Issue

Vol. 12 No. 3 (2011): Current Problems of Psychiatry

Section

Artykuły

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