Ultrastructural evaluation of renal tubular epithelial cells of the rats’ kidneys after anthracyclines therapy

Authors

  • Agnieszka Pedrycz Department of Histology and Embryology with Laboratory of Experimental Cytology, Medical University of Lublin, 20-080 Lublin, Poland Author
  • Piotr Siermontowski Military Institute of Medicine, Maritime & Hyperbaric Medicine Department, Gdynia Poland Author
  • Dorota Polz Departament of Virology, Medical University of Lublin, Lublin, Poland Author
  • Janusz Kocki Laboratory of Clinical Genetics, Medical University of Lublin, 20-080 Lublin, Poland Author
  • Agnieszka Puchalska-Kosiecz Ith Department Oncologic Gynecology et Gynecology Medical University of Lublin, Poland Author

Keywords:

ultrastructure of renal tubular epithelial cells, semi thin slides, apoptosis, adriamycin

Abstract

Anthracyclines are antineooplastic antibiotics. In the present study Adriamycin (ADR) induced apoptosis of renal tubular epithelial cells. The study material consisted of Wistar female rats, which were divided into 2 control and 2 experimental groups. Experimental groups: rats treated with a single intraperitoneal dose of Adriamycin - 5 mg/kg body weight and decapitated after 4 (gr. I) and 7 (gr. III) weeks. Control groups: rats treated with a single intraperitoneal dose of 0.9% NaCl- 0.5 ml and decapitated after 4 (gr. II) and 7 (gr. IV) weeks. Semi-thin - 0.5-0.7 μm and ultrathin - 60 nm slideswere prepared from left kidneys segments taken from the rats. Under electron and light microscope using semi-thin slides we observed apoptosis of kidney’ cells. In experimental groups a large number of cells showed damaged pyknotic nuclei of reduced perimeter, changed shape and dark blue-black in semi-thin specimens. Naked pyknotic nuclei were visible in the renal tubular lumen. Those changes increased in the time of the experiment.

References

1. Kerr J.R.F., Wyllie A.H., Currie A.R. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br. J. Cancer., 1972; 26: 139-257.

2. Lockshin R., Zakeri Z., Tilly J. When cells die. A comprehensive evaluation of apoptosis and programmed cell death. Wiley-Liss: 1998.

3. Pedrycz A., Kosiecz D., Drelich G., Ossowska B. Over-expression of biomarkers of environmental stress in renal epithelial cells resulting from proapoptotic activity of adriamycin: An immunohistochemical assessment. Curr. Probl. Psychiatry, 2010; 1: 161-165.

4. Dash P.R., Cartwiright J.E., Baker P.N., Johnstone A.P., Whitley G.S. Nitric oxide protects human extravillous trophoblast cells from apoptosis by a cylic GMP-depent mchanism and independly of caspase 3 nitrosilation. Ex. Cell. Res., 2003; 287: 314-24.

5. Johnstone R.W., Ruefli A.A., Lowe S.W. Apoptosis: a link between cancer genetics and chemiotherapy. Cell, 2002; 108: 153-164

6. Hengartner M.O. The biochemistry of apoptosis. Nature, 2000; 407: 770-776.

7. Jablonski E.M., Hughes F.M. Jr. The potential role of Caveolin-1 in Inhibition of Aquaporins during the AVD. Biol. Cell., 2006; 98: 33-42.

8. Gong Y.H., Guo X., Zhang X.Q. Immunoelectron microscopic studies on the process of tumor cytolysis mediated by lymphokine activated NK cells. Chung Hua Ping Li Hsueh Tsa Chih, 1994; 23: 17-19.

9. Fadok V., Bratton D.M., Rose A., Pearson R., Alan B., Ezekewitz P.M., Henson P.M. A receptor for fosfatidyloserine – specific clearance of apoptotic cells. Nature, 2000; 405: 85-90.

10. Bagnato A., Bianchi C., Caputo A., Silvestrini B., Florich A. Enhancing effect of loniamide on the inhibition of mitochondrial respiration by adriamycin. Anticancer Res., 1987; 7: 799-802.

11. Oz E., Erbas D., Surucu H.S., Duzgun E. Prevention of doxorubicininduced cardiotoxicity by melatonin. Mol. Cell Biochem., 2006; 282: 31-37.

12. Serafino A., Sinibaldi-Vallebona P., Lazzarino G., Tavazzi B., Di Pierro D., Rasi G., Ravagnan G. Modifications of mitochondria in human tumor cells during anthracycline-induced apoptosis. Anticancer Res., 2000; 20: 3383-3394.

13. Zeidan Q., Strauss M., Porras N., Anselmi G. Differential long-term subcellular responses in heart and liver to adriamycin stress. Exogenous L-carnitine cardiac and hepatic protection. J. Submicrosc. Cytol. Pathol., 2002; 34: 315-21.

14. Grzanka D., Domaniewski J., Grzanka A. Effect of doxorubicin on actin reorganization in Chinese hamster ovary cells. Neoplasma, 2005; 52: 46-51.

15. Rudolph R., Woodward M., Hurn I. Ultrastructure of doxorubicin (adriamycin)-induced skin ulcers in rats. Cancer Res., 1979; 39: 3689-3693.

16. Skomra D., Chibowski D. Assessment of Daunorubicin nephrotoxicity and effect of tocopherol and acsorbic acid on lesions induced in rat’s kidneys. Part I. histological and histochemical and biochemical studies. Ann. Univ. Mariae Curie Sklodowska, 1993; 98: 181-189.

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Published

2011-05-19

Issue

Vol. 12 No. 1 (2011): Current Problems of Psychiatry

Section

Artykuły

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